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PMID:12426334

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Citation

Gallagher, LA, McKnight, SL, Kuznetsova, MS, Pesci, EC and Manoil, C (2002) Functions required for extracellular quinolone signaling by Pseudomonas aeruginosa. J. Bacteriol. 184:6472-80

Abstract

A set of 30 mutants exhibiting reduced production of the phenazine poison pyocyanin were isolated following transposon mutagenesis of Pseudomonas aeruginosa PAO1. The mutants could be subdivided into those with defects in the primary phenazine biosynthetic pathway and those with more pleiotropic defects. The largest set of pleiotropic mutations blocked the production of the extracellular Pseudomonas quinolone signal (PQS), a molecule required for the synthesis of secondary metabolites and extracellular enzymes. Most of these pqs mutations affected genes which appear to encode PQS biosynthetic functions, although a transcriptional regulator and an apparent response effector were also represented. Two of the genes required for PQS synthesis (phnA and phnB) had previously been assumed to encode phenazine biosynthetic functions. The transcription of one of the genes required for PQS synthesis (PA2587/pqsH) was regulated by the LasI/R quorum-sensing system, thereby linking quorum sensing and PQS regulation. Others of the pleiotropic phenazine-minus mutations appear to inactivate novel components of the quorum-sensing regulatory network, including one regulator (np20) previously shown to be required for virulence in neutropenic mice.

Links

PubMed PMC135424

Keywords

Animals; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Caenorhabditis elegans/growth & development; DNA Transposable Elements; Gene Expression Regulation, Bacterial; Genetic Complementation Test; Mutagenesis, Insertional; Mutation; Pseudomonas aeruginosa/genetics; Pseudomonas aeruginosa/growth & development; Pseudomonas aeruginosa/metabolism; Pyocyanine/biosynthesis; Quinolones/metabolism; Signal Transduction

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

PSEAE:PQSE

acts_upstream_of_or_within

GO:0044550: secondary metabolite biosynthetic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

PSEAE:PQSD

acts_upstream_of_or_within

GO:0044550: secondary metabolite biosynthetic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

PSEAE:Q9HT74

acts_upstream_of_or_within

GO:1900376: regulation of secondary metabolite biosynthetic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

PSEAE:PQSC

acts_upstream_of_or_within

GO:0044550: secondary metabolite biosynthetic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

PSEAE:PQSB

acts_upstream_of_or_within

GO:0044550: secondary metabolite biosynthetic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

PSEAE:PQSA

acts_upstream_of_or_within

GO:0044550: secondary metabolite biosynthetic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

PSEAE:PQSD

involved_in

GO:0044550: secondary metabolite biosynthetic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

PSEAE:PQSA

involved_in

GO:0044550: secondary metabolite biosynthetic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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