PMID:12426334

Citation	Gallagher, LA, McKnight, SL, Kuznetsova, MS, Pesci, EC and Manoil, C (2002) Functions required for extracellular quinolone signaling by Pseudomonas aeruginosa. J. Bacteriol. 184:6472-80
Abstract	A set of 30 mutants exhibiting reduced production of the phenazine poison pyocyanin were isolated following transposon mutagenesis of Pseudomonas aeruginosa PAO1. The mutants could be subdivided into those with defects in the primary phenazine biosynthetic pathway and those with more pleiotropic defects. The largest set of pleiotropic mutations blocked the production of the extracellular Pseudomonas quinolone signal (PQS), a molecule required for the synthesis of secondary metabolites and extracellular enzymes. Most of these pqs mutations affected genes which appear to encode PQS biosynthetic functions, although a transcriptional regulator and an apparent response effector were also represented. Two of the genes required for PQS synthesis (phnA and phnB) had previously been assumed to encode phenazine biosynthetic functions. The transcription of one of the genes required for PQS synthesis (PA2587/pqsH) was regulated by the LasI/R quorum-sensing system, thereby linking quorum sensing and PQS regulation. Others of the pleiotropic phenazine-minus mutations appear to inactivate novel components of the quorum-sensing regulatory network, including one regulator (np20) previously shown to be required for virulence in neutropenic mice.
Links	PubMed PMC135424
Keywords	Animals; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Caenorhabditis elegans/growth & development; DNA Transposable Elements; Gene Expression Regulation, Bacterial; Genetic Complementation Test; Mutagenesis, Insertional; Mutation; Pseudomonas aeruginosa/genetics; Pseudomonas aeruginosa/growth & development; Pseudomonas aeruginosa/metabolism; Pyocyanine/biosynthesis; Quinolones/metabolism; Signal Transduction
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
PSEAE:PQSE	acts_upstream_of_or_within	GO:0044550: secondary metabolite biosynthetic process	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
PSEAE:PQSD	acts_upstream_of_or_within	GO:0044550: secondary metabolite biosynthetic process	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
PSEAE:Q9HT74	acts_upstream_of_or_within	GO:1900376: regulation of secondary metabolite biosynthetic process	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
PSEAE:PQSC	acts_upstream_of_or_within	GO:0044550: secondary metabolite biosynthetic process	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
PSEAE:PQSB	acts_upstream_of_or_within	GO:0044550: secondary metabolite biosynthetic process	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
PSEAE:PQSA	acts_upstream_of_or_within	GO:0044550: secondary metabolite biosynthetic process	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
PSEAE:PQSD	involved_in	GO:0044550: secondary metabolite biosynthetic process	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
PSEAE:PQSA	involved_in	GO:0044550: secondary metabolite biosynthetic process	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete

References

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