GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:12393877

From GONUTS
Jump to: navigation, search
Citation

Steiglitz, BM, Keene, DR and Greenspan, DS (2002) PCOLCE2 encodes a functional procollagen C-proteinase enhancer (PCPE2) that is a collagen-binding protein differing in distribution of expression and post-translational modification from the previously described PCPE1. J. Biol. Chem. 277:49820-30

Abstract

The procollagen COOH-terminal proteinase enhancer (PCPE) is a glycoprotein that binds the COOH-terminal propeptide of type I procollagen and potentiates its cleavage by procollagen C-proteinases, such as bone morphogenetic protein-1 (BMP-1). Recently, sequencing of a human expressed sequence tag, which maps near the primary open angle glaucoma region on chromosome 3q21, showed it to encode a novel protein with only 43% identity with PCPE but with a similar domain structure. Here we show this novel protein to be a functional procollagen COOH-terminal proteinase enhancer with activity comparable with that of PCPE and thus propose the designations PCPE2 and PCPE1, respectively. PCPE2 is shown to have a much more limited distribution of expression than does PCPE1, with strong expression primarily in nonossified cartilage in developing tissues and at high levels in the adult heart. PCPE2 is shown to be a glycoprotein that differs markedly in the nature of its glycosylation from that of PCPE1. PCPE2 is also shown to have markedly stronger affinity for heparin than PCPE1, which may account for higher affinities for cell layers. Unexpectedly, both PCPE1 and PCPE2 were found to be collagen-binding proteins, capable of binding at multiple sites on the triple helical portions of fibrillar collagens and also capable of competing for such binding with procollagen C-proteinases. The latter observations may provide insights into the ways PCPEs affect the kinetics of the C-proteinase reaction and into the physical interactions that occur between procollagen C-proteinases and their substrates.

Links

PubMed Online version:10.1074/jbc.M209891200

Keywords

Amino Acid Sequence; Animals; Binding Sites; Blotting, Northern; Blotting, Western; Cell Line; Collagen/chemistry; Collagen/metabolism; DNA, Complementary/metabolism; Embryo, Mammalian/metabolism; Enhancer Elements, Genetic; Extracellular Matrix Proteins; Glycoproteins/biosynthesis; Glycoproteins/chemistry; Glycoproteins/metabolism; Glycoproteins/physiology; Glycosylation; Heparin/metabolism; Humans; In Situ Hybridization; Lectins/metabolism; Mice; Microscopy, Electron; Molecular Sequence Data; Procollagen/chemistry; Protein Binding; Protein Processing, Post-Translational; Protein Structure, Tertiary; RNA/metabolism; Recombinant Proteins/metabolism; Sequence Homology, Amino Acid; Tissue Distribution

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:BMP1

enables

GO:0008233: peptidase activity

ECO:0000266: sequence orthology evidence used in manual assertion

UniProtKB:P13497

F

Seeded From UniProt

complete

HUMAN:PCOC1

enables

GO:0005518: collagen binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:PCOC1

enables

GO:0008201: heparin binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:PCOC1

enables

GO:0016504: peptidase activator activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

MOUSE:PCOC1

enables

GO:0005518: collagen binding

ECO:0000266: sequence orthology evidence used in manual assertion

UniProtKB:Q15113

F

Seeded From UniProt

complete

MOUSE:PCOC1

enables

GO:0008201: heparin binding

ECO:0000266: sequence orthology evidence used in manual assertion

UniProtKB:Q15113

F

Seeded From UniProt

complete

MOUSE:PCOC1

enables

GO:0016504: peptidase activator activity

ECO:0000266: sequence orthology evidence used in manual assertion

UniProtKB:Q15113

F

Seeded From UniProt

complete

MOUSE:TLL1

enables

GO:0005518: collagen binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

MOUSE:PCOC2

enables

GO:0005518: collagen binding

ECO:0000266: sequence orthology evidence used in manual assertion

UniProtKB:Q9UKZ9

F

Seeded From UniProt

complete

MOUSE:PCOC2

enables

GO:0008201: heparin binding

ECO:0000266: sequence orthology evidence used in manual assertion

UniProtKB:Q9UKZ9

F

Seeded From UniProt

complete

MOUSE:PCOC2

enables

GO:0016504: peptidase activator activity

ECO:0000266: sequence orthology evidence used in manual assertion

UniProtKB:Q9UKZ9

F

Seeded From UniProt

complete

HUMAN:PCOC2

enables

GO:0005518: collagen binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:PCOC2

enables

GO:0008201: heparin binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:PCOC2

enables

GO:0016504: peptidase activator activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:BMP1

enables

GO:0008233: peptidase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.