PMID:12388544

Citation	Han, YH, Chung, CY, Wessels, D, Stephens, S, Titus, MA, Soll, DR and Firtel, RA (2002) Requirement of a vasodilator-stimulated phosphoprotein family member for cell adhesion, the formation of filopodia, and chemotaxis in dictyostelium. J. Biol. Chem. 277:49877-87
Abstract	We have examined the function of a member of the vasodilator-stimulated phosphoprotein family of proteins (DdVASP) in Dictyostelium. Ddvasp null cells lack filopodia, whereas targeting DdVASP to the plasma membrane with a myristoyl tag results in a significant increase in filopodia. The proline-rich domain-Ena/VASP homology 2 structure is required for both actin polymerization activity and filopodia formation. Ddvasp null cells exhibit a chemotaxis defect, which appears to be due to a defect in the ability of the cells to properly adhere to the substratum and to suppress lateral pseudopod extension. We demonstrate that during chemotaxis, the anterior approximately 50% of the cell lifts from the substratum and remains elevated for up to 1 min. These defects lead to a significant decrease in chemotaxis efficiency. DdVASP localizes to the leading edge in migrating cells and to the tips of filopodia. In addition, Ddvasp null cells have a defect in particle adhesion but internalize particles normally. Our results provide new insights into the function of DdVASP in controlling the actin cytoskeleton during chemotaxis and filopodia formation.
Links	PubMed Online version:10.1074/jbc.M209107200
Keywords	Actins/metabolism; Amino Acid Sequence; Animals; Cell Adhesion; Cell Adhesion Molecules/metabolism; Cell Adhesion Molecules/physiology; Chemotaxis; Cloning, Molecular; Cyclic AMP/metabolism; Cytoskeleton/metabolism; Dictyostelium/metabolism; Dictyostelium/physiology; Gene Deletion; Green Fluorescent Proteins; Humans; Kinetics; Luminescent Proteins/metabolism; Microfilament Proteins; Models, Biological; Molecular Sequence Data; Movement; Peptides/chemistry; Phagocytosis; Phosphoproteins/metabolism; Phosphoproteins/physiology; Precipitin Tests; Proline/chemistry; Protein Binding; Protein Structure, Tertiary; Pseudopodia/metabolism; Recombinant Fusion Proteins/metabolism; Sequence Homology, Amino Acid; Time Factors; Vasodilator Agents/pharmacology
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
DICDI:VASP	acts_upstream_of_or_within	GO:0030041: actin filament polymerization	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
DICDI:VASP	located_in	GO:0030175: filopodium	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
DICDI:VASP	acts_upstream_of_or_within	GO:0031589: cell-substrate adhesion	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
DICDI:VASP	located_in	GO:0005829: cytosol	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
DICDI:VASP	acts_upstream_of_or_within	GO:0007015: actin filament organization	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
DICDI:VASP	located_in	GO:0031252: cell leading edge	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
DICDI:VASP	located_in	GO:0005938: cell cortex	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
DICDI:VASP	acts_upstream_of_or_within	GO:0050922: negative regulation of chemotaxis	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
DICDI:VASP	acts_upstream_of_or_within	GO:0046847: filopodium assembly	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete

References

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