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PMID:12388544

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Citation

Han, YH, Chung, CY, Wessels, D, Stephens, S, Titus, MA, Soll, DR and Firtel, RA (2002) Requirement of a vasodilator-stimulated phosphoprotein family member for cell adhesion, the formation of filopodia, and chemotaxis in dictyostelium. J. Biol. Chem. 277:49877-87

Abstract

We have examined the function of a member of the vasodilator-stimulated phosphoprotein family of proteins (DdVASP) in Dictyostelium. Ddvasp null cells lack filopodia, whereas targeting DdVASP to the plasma membrane with a myristoyl tag results in a significant increase in filopodia. The proline-rich domain-Ena/VASP homology 2 structure is required for both actin polymerization activity and filopodia formation. Ddvasp null cells exhibit a chemotaxis defect, which appears to be due to a defect in the ability of the cells to properly adhere to the substratum and to suppress lateral pseudopod extension. We demonstrate that during chemotaxis, the anterior approximately 50% of the cell lifts from the substratum and remains elevated for up to 1 min. These defects lead to a significant decrease in chemotaxis efficiency. DdVASP localizes to the leading edge in migrating cells and to the tips of filopodia. In addition, Ddvasp null cells have a defect in particle adhesion but internalize particles normally. Our results provide new insights into the function of DdVASP in controlling the actin cytoskeleton during chemotaxis and filopodia formation.

Links

PubMed Online version:10.1074/jbc.M209107200

Keywords

Actins/metabolism; Amino Acid Sequence; Animals; Cell Adhesion; Cell Adhesion Molecules/metabolism; Cell Adhesion Molecules/physiology; Chemotaxis; Cloning, Molecular; Cyclic AMP/metabolism; Cytoskeleton/metabolism; Dictyostelium/metabolism; Dictyostelium/physiology; Gene Deletion; Green Fluorescent Proteins; Humans; Kinetics; Luminescent Proteins/metabolism; Microfilament Proteins; Models, Biological; Molecular Sequence Data; Movement; Peptides/chemistry; Phagocytosis; Phosphoproteins/metabolism; Phosphoproteins/physiology; Precipitin Tests; Proline/chemistry; Protein Binding; Protein Structure, Tertiary; Pseudopodia/metabolism; Recombinant Fusion Proteins/metabolism; Sequence Homology, Amino Acid; Time Factors; Vasodilator Agents/pharmacology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DICDI:VASP

acts_upstream_of_or_within

GO:0030041: actin filament polymerization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:VASP

located_in

GO:0030175: filopodium

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DICDI:VASP

acts_upstream_of_or_within

GO:0031589: cell-substrate adhesion

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:VASP

located_in

GO:0005829: cytosol

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DICDI:VASP

acts_upstream_of_or_within

GO:0007015: actin filament organization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:VASP

located_in

GO:0031252: cell leading edge

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DICDI:VASP

located_in

GO:0005938: cell cortex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DICDI:VASP

acts_upstream_of_or_within

GO:0050922: negative regulation of chemotaxis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:VASP

acts_upstream_of_or_within

GO:0046847: filopodium assembly

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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