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PMID:12376626

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Citation

Charrier, B, Champion, A, Henry, Y and Kreis, M (2002) Expression profiling of the whole Arabidopsis shaggy-like kinase multigene family by real-time reverse transcriptase-polymerase chain reaction. Plant Physiol. 130:577-90

Abstract

The recent publication of the complete sequence of the Arabidopsis genome allowed us to identify and characterize the last two members of the SHAGGY-like kinase (AtSK) gene family. As a result, the study of the overall spatio-temporal organization of the whole AtSK family in Arabidopsis has become an achievable and necessary aim to understand the role of each SHAGGY-like kinase during plant development. An analysis of the transcript level of the 10 members of the family has been performed using the technique of real-time quantitative reverse transcriptase-polymerase chain reaction. Transcript levels in several organs, under different growth conditions, were analyzed. To calibrate the results obtained, a number of other genes, such as those coding for the two MAP3Kepsilons and the two MAP4Kalphas, as well as the stress response marker RD29A; the small subunit of the Rubisco photosynthetic enzyme Ats1A; the MEDEA chromatin remodeling factor; and the SCARECROW, ASYMMETRIC LEAVES 1, and SUPERMAN transcription factors all involved in key steps of plant development were used. The analysis of our data revealed that eight of the 10 genes of the AtSK family displayed a pseudo-constitutive expression pattern at the organ level. Conversely, AtSK13 responded to osmotic changes and saline treatment, whereas AtSK31 was flower specific and responded to osmotic changes and darkness.

Links

PubMed PMC166588 Online version:10.1104/pp.009175

Keywords

Adaptation, Physiological/drug effects; Adaptation, Physiological/genetics; Adaptation, Physiological/radiation effects; Arabidopsis/enzymology; Arabidopsis/genetics; Arabidopsis/growth & development; Arabidopsis Proteins/drug effects; Arabidopsis Proteins/genetics; Arabidopsis Proteins/radiation effects; Gene Expression Profiling/methods; Gene Expression Regulation, Developmental/drug effects; Gene Expression Regulation, Developmental/radiation effects; Gene Expression Regulation, Enzymologic/drug effects; Gene Expression Regulation, Enzymologic/radiation effects; Gene Expression Regulation, Plant/drug effects; Gene Expression Regulation, Plant/radiation effects; Glycogen Synthase Kinase 3/analogs & derivatives; Glycogen Synthase Kinase 3/drug effects; Glycogen Synthase Kinase 3/genetics; Glycogen Synthase Kinase 3/radiation effects; Light; Osmotic Pressure/drug effects; Phylogeny; Polyethylene Glycols/pharmacology; Reverse Transcriptase Polymerase Chain Reaction/methods; Sodium Chloride/pharmacology; Stress, Mechanical; Transcription Factors/genetics; Water/pharmacology; Water/physiology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:F4HPR9

acts_upstream_of_or_within

GO:0006972: hyperosmotic response

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:F4HPR9

acts_upstream_of_or_within

GO:0009651: response to salt stress

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:F4HPR9

acts_upstream_of_or_within

GO:0009611: response to wounding

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:F4I9N7

acts_upstream_of_or_within

GO:0006972: hyperosmotic response

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:F4I9N7

acts_upstream_of_or_within

GO:0009651: response to salt stress

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:MEDEA

acts_upstream_of_or_within

GO:0009646: response to absence of light

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:CSN1

acts_upstream_of_or_within

GO:0006972: hyperosmotic response

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:CSN1

acts_upstream_of_or_within

GO:0009651: response to salt stress

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:CSN1

acts_upstream_of_or_within

GO:0009646: response to absence of light

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:KSG5

acts_upstream_of_or_within

GO:0006972: hyperosmotic response

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:KSG5

acts_upstream_of_or_within

GO:0009651: response to salt stress

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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