GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:12213835
| Citation |
Rogers, SL, Rogers, GC, Sharp, DJ and Vale, RD (2002) Drosophila EB1 is important for proper assembly, dynamics, and positioning of the mitotic spindle. J. Cell Biol. 158:873-84 |
|---|---|
| Abstract |
EB1 is an evolutionarily conserved protein that localizes to the plus ends of growing microtubules. In yeast, the EB1 homologue (BIM1) has been shown to modulate microtubule dynamics and link microtubules to the cortex, but the functions of metazoan EB1 proteins remain unknown. Using a novel preparation of the Drosophila S2 cell line that promotes cell attachment and spreading, we visualized dynamics of single microtubules in real time and found that depletion of EB1 by RNA-mediated inhibition (RNAi) in interphase cells causes a dramatic increase in nondynamic microtubules (neither growing nor shrinking), but does not alter overall microtubule organization. In contrast, several defects in microtubule organization are observed in RNAi-treated mitotic cells, including a drastic reduction in astral microtubules, malformed mitotic spindles, defocused spindle poles, and mispositioning of spindles away from the cell center. Similar phenotypes were observed in mitotic spindles of Drosophila embryos that were microinjected with anti-EB1 antibodies. In addition, live cell imaging of mitosis in Drosophila embryos reveals defective spindle elongation and chromosomal segregation during anaphase after antibody injection. Our results reveal crucial roles for EB1 in mitosis, which we postulate involves its ability to promote the growth and interactions of microtubules within the central spindle and at the cell cortex. |
| Links |
PubMed PMC2173155 Online version:10.1083/jcb.200202032 |
| Keywords |
Anaphase; Animals; Cell Line; Chromosome Segregation; Drosophila melanogaster/cytology; Drosophila melanogaster/embryology; Drosophila melanogaster/metabolism; Interphase; Microscopy, Fluorescence; Microtubule-Associated Proteins/deficiency; Microtubule-Associated Proteins/metabolism; Microtubules/metabolism; Mitosis; Mitotic Spindle Apparatus/metabolism; Time Factors |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| edit table |
See also
References
See Help:References for how to manage references in GONUTS.
