PMID:12198165

Citation	Zhou, Y, Santoro, R and Grummt, I (2002) The chromatin remodeling complex NoRC targets HDAC1 to the ribosomal gene promoter and represses RNA polymerase I transcription. EMBO J. 21:4632-40
Abstract	Mammalian chromatin remodeling complexes are involved in both activation and repression of transcription. Here, we show that NoRC, a SNF2h- containing nucleolar chromatin remodeling complex, represses ribosomal gene transcription. NoRC-mediated rDNA silencing was alleviated by trichostatin A, indicating that histone deacetylation is causally involved in silencing. Chromatin immunoprecipitation experiments demonstrate that overexpression of TIP5, the large subunit of NoRC, mediates deacetylation of nucleosomes in the vicinity of the rDNA promoter. Protein-protein interaction assays reveal association of TIP5 with the histone deacetylase HDAC1 in vivo and in vitro. Deletion of the C-terminal PHD finger and bromodomain abolishes the interaction of TIP5 and HDAC1, and abrogates transcriptional repression. The results suggest that NoRC silences the rDNA locus by targeting the SIN3 corepressor complex to the rDNA promoter, thereby establishing a repressed chromatin structure.
Links	PubMed PMC126197
Keywords	3T3 Cells; Acetylation; Amino Acid Motifs; Animals; Cell Line; Chromatin/metabolism; Chromosomal Proteins, Non-Histone/chemistry; Chromosomal Proteins, Non-Histone/physiology; DNA, Ribosomal/genetics; Gene Silencing; Genes, rRNA; Histone Deacetylase 1; Histone Deacetylases/metabolism; Histones/metabolism; Macromolecular Substances; Mice; Multienzyme Complexes/metabolism; Nucleosomes/metabolism; Promoter Regions, Genetic; Protein Interaction Mapping; Protein Processing, Post-Translational; Protein Structure, Tertiary; RNA Polymerase I/antagonists & inhibitors; Recombinant Fusion Proteins/physiology; Sin3 Histone Deacetylase and Corepressor Complex; Trans-Activators/physiology; Transcription, Genetic
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
MOUSE:HDAC1	enables	GO:0005515: protein binding	ECO:0000353: physical interaction evidence used in manual assertion	UniProtKB:Q91YE5	F		Seeded From UniProt	complete
MOUSE:SIN3A	enables	GO:0005515: protein binding	ECO:0000353: physical interaction evidence used in manual assertion	UniProtKB:Q91YE5	F		Seeded From UniProt	complete
MOUSE:BAZ2A	located_in	GO:0033553: rDNA heterochromatin	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
MOUSE:BAZ2A	involved_in	GO:0000183: rDNA heterochromatin assembly	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
MOUSE:BAZ2A	enables	GO:0005515: protein binding	ECO:0000353: physical interaction evidence used in manual assertion	UniProtKB:Q60520	F		Seeded From UniProt	complete
MOUSE:BAZ2A	enables	GO:0005515: protein binding	ECO:0000353: physical interaction evidence used in manual assertion	UniProtKB:O09106	F		Seeded From UniProt	complete
MOUSE:BAZ2A	involved_in	GO:0031065: positive regulation of histone deacetylation	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
MOUSE:SMCA5	involved_in	GO:0031065: positive regulation of histone deacetylation	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
MOUSE:SMCA5	involved_in	GO:0000183: rDNA heterochromatin assembly	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
MOUSE:BAZ2A	part_of	GO:0033553: rDNA heterochromatin	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
MOUSE:BAZ2A	involved_in	GO:0016575: histone deacetylation	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
MOUSE:BAZ2A	involved_in	GO:0000183: chromatin silencing at rDNA	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete

References

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