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PMID:12193658

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Citation

Noma, K and Grewal, SI (2002) Histone H3 lysine 4 methylation is mediated by Set1 and promotes maintenance of active chromatin states in fission yeast. Proc. Natl. Acad. Sci. U.S.A. 99 Suppl 4:16438-45

Abstract

Methylation of histone H3 at lysine 4 (H3 Lys-4) or lysine 9 (H3 Lys-9) is known to define active and silent chromosomal domains respectively from fission yeast to humans. However, in budding yeast, H3 Lys-4 methylation is also necessary for silent chromatin assembly at telomeres and ribosomal DNA. Here we demonstrate that deletion of set1, which encodes a protein containing an RNA recognition motif at its amino terminus and a SET domain at the carboxy terminus, abolishes H3 Lys-4 methylation in fission yeast. Unlike in budding yeast, Set1-mediated H3 Lys-4 methylation is not required for heterochromatin assembly at the silent mating-type region and centromeres in fission yeast. Our analysis suggests that H3 Lys-4 methylation is a stable histone modification present throughout the cell cycle, including mitosis. The loss of H3 Lys-4 methylation in set1Delta cells is correlated with a decrease in histone H3 acetylation levels, suggesting a mechanistic link between H3 Lys-4 methylation and acetylation of the H3 tail. We suggest that methylation of H3 Lys-4 primarily acts in the maintenance of transcriptionally poised euchromatic domains, and that this modification is dispensable for heterochromatin formation in fission yeast, which instead utilizes H3 Lys-9 methylation.

Links

PubMed PMC139906 Online version:10.1073/pnas.182436399

Keywords

Cell Cycle; Chromatin/metabolism; Fluorescent Antibody Technique; Histones/chemistry; Histones/metabolism; Lysine/metabolism; Methylation; Schizosaccharomyces/cytology; Schizosaccharomyces/genetics; Schizosaccharomyces/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

SCHPO:SET1

enables

GO:0042800: histone methyltransferase activity (H3-K4 specific)

ECO:0000315: mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete

SCHPO:SET1

involved_in

GO:0051568: histone H3-K4 methylation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

Notes

See also

References

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