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PMID:12167649

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Citation

Young, ET, Kacherovsky, N and Van Riper, K (2002) Snf1 protein kinase regulates Adr1 binding to chromatin but not transcription activation. J. Biol. Chem. 277:38095-103

Abstract

The yeast transcriptional activator Adr1 controls the expression of genes required for ethanol, glycerol, and fatty acid utilization. We show that Adr1 acts directly on the promoters of ADH2, ACS1, GUT1, CTA1, and POT1 using chromatin immunoprecipitation assays. The yeast homolog of the AMP-activated protein kinase, Snf1, promotes Adr1 chromatin binding in the absence of glucose, and the protein phosphatase complex, Glc7.Reg1, represses its binding in the presence of glucose. A post-translational process is implicated in the regulation of Adr1 binding activity. Chromatin binding by Adr1 is not the only step in ADH2 transcription that is regulated by glucose repression. Adr1 can bind to chromatin in repressed conditions in the presence of hyperacetylated histones. To study steps subsequent to promoter binding we utilized miniAdr1 transcription factors to characterize Adr1-dependent transcription in vitro. Yeast nuclear extracts prepared from glucose-repressed and glucose-derepressed cells are equally capable of supporting miniAdr1-dependent transcription and pre-initiation complex formation. Nuclear extracts prepared from a snf1 mutant support miniAdr1-dependent transcription but are partially defective in the formation of pre-initiation complexes with Mediator components being particularly depleted. We conclude that Snf1 regulates Adr1-dependent transcription primarily at the level of chromatin binding.

Links

PubMed Online version:10.1074/jbc.M206158200

Keywords

Chromatin/metabolism; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Gene Expression Regulation, Fungal; Genes, Reporter; Glucose/metabolism; Promoter Regions, Genetic; Protein Binding; Protein-Serine-Threonine Kinases/genetics; Protein-Serine-Threonine Kinases/metabolism; Saccharomyces cerevisiae/physiology; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism; Transcription Factors/genetics; Transcription Factors/metabolism; Transcriptional Activation

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:PP12

involved_in

GO:0006109: regulation of carbohydrate metabolic process

ECO:0000316: genetic interaction evidence used in manual assertion

P

Seeded From UniProt

Missing: with/from

YEAST:PP12

involved_in

GO:0006109: regulation of carbohydrate metabolic process

ECO:0000353: physical interaction evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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