GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com


Jump to: navigation, search

Lin, HK, Wang, L, Hu, YC, Altuwaijri, S and Chang, C (2002) Phosphorylation-dependent ubiquitylation and degradation of androgen receptor by Akt require Mdm2 E3 ligase. EMBO J. 21:4037-48


The androgen receptor (AR) controls several biological functions including prostate cell growth and apoptosis. However, the mechanism by which AR maintains its stability to function properly remains largely unknown. Here we show that Akt and Mdm2 form a complex with AR and promote phosphorylation-dependent AR ubiquitylation, resulting in AR degradation by the proteasome. The effect of Akt on AR ubiquitylation and degradation is markedly impaired in a Mdm2-null cell line compared with the wild-type cell line, suggesting that Mdm2 is involved in Akt-mediated AR ubiquitylation and degradation. Furthermore, we demonstrate that the E3 ligase activity of Mdm2 and phosphorylation of Mdm2 by Akt are essential for Mdm2 to affect AR ubiquitylation and degradation. These results suggest that phosphorylation-dependent AR ubiquitylation and degradation by Akt require the involvement of Mdm2 E3 ligase activity, a novel mechanism that provides insight into how AR is targeted for degradation.


PubMed PMC126152


Animals; Cell Line/metabolism; Cercopithecus aethiops; Colony-Forming Units Assay; Enzyme Activation; Fibroblasts/metabolism; Ligases/chemistry; Ligases/physiology; Macromolecular Substances; Mice; Nuclear Proteins; Peptide Hydrolases/metabolism; Phosphatidylinositol 3-Kinases/metabolism; Phosphorylation; Proteasome Endopeptidase Complex; Protein Interaction Mapping; Protein Processing, Post-Translational/physiology; Protein-Serine-Threonine Kinases; Proto-Oncogene Proteins/chemistry; Proto-Oncogene Proteins/physiology; Proto-Oncogene Proteins c-akt; Proto-Oncogene Proteins c-mdm2; Rats; Receptors, Androgen/chemistry; Receptors, Androgen/metabolism; Recombinant Fusion Proteins/physiology; Transcription, Genetic; Ubiquitin/metabolism; Ubiquitin-Protein Ligases



Gene product Qualifier GO ID GO term name Evidence Code with/from Aspect Notes Status

See also


See Help:References for how to manage references in GONUTS.