GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:12127992

From GONUTS
Jump to: navigation, search
Citation

I Bannon, D, Portnoy, ME, Olivi, L, Lees, PS, Culotta, VC and Bressler, JP (2002) Uptake of lead and iron by divalent metal transporter 1 in yeast and mammalian cells. Biochem. Biophys. Res. Commun. 295:978-84

Abstract

Although the divalent metal transporter (DMT1) was suggested to transport a wide range of metals in Xenopus oocytes, recent studies in other models have provided contrasting results. Here, we provide direct evidence demonstrating that DMT1 expressed in yeast mutants defective for high affinity iron transport facilitates the transport of iron with an 'apparent K(m)' of approximately 1.2 microM, and transport of lead with an 'apparent K(m)' of approximately 1.8 microM. DMT1-dependent lead transport was H(+)-dependent and was inhibited by iron. Human embryonic kidney fibroblasts (HEK293 cells) overexpressing DMT1 also showed a higher uptake of lead than HEK293 cells without overexpressing DMT1. These results show that DMT1 transports lead and iron with similar affinity in a yeast model suggesting that DMT1 is a transporter for lead.

Links

PubMed

Keywords

Animals; Carrier Proteins/metabolism; Cation Transport Proteins; Cell Line; DNA, Complementary/metabolism; Dose-Response Relationship, Drug; Fibroblasts/metabolism; Humans; Hydrogen/metabolism; Hydrogen-Ion Concentration; Iron/metabolism; Iron/pharmacokinetics; Iron-Binding Proteins; Kinetics; Lead/metabolism; Lead/pharmacokinetics; Mutation; Plasmids/metabolism; Rats; Saccharomyces cerevisiae/metabolism; Time Factors

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:NRAM2

GO:0015094: lead ion transmembrane transporter activity

ECO:0000314:

F

complete

HUMAN:NRAM2

GO:0015692: lead ion transport

ECO:0000314:

P

complete

HUMAN:NRAM2

enables

GO:0015094: lead ion transmembrane transporter activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:NRAM2

involved_in

GO:0015692: lead ion transport

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:Q8IUD7

involved_in

GO:0015692: lead ion transport

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:Q8IUD7

enables

GO:0015094: lead ion transmembrane transporter activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.