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PMID:12090754
| Citation |
Uemura, A, Takizawa, T, Ochiai, W, Yanagisawa, M, Nakashima, K and Taga, T (2002) Cardiotrophin-like cytokine induces astrocyte differentiation of fetal neuroepithelial cells via activation of STAT3. Cytokine 18:1-7 |
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| Abstract |
Cardiotrophin-like cytokine (CLC), also known as novel neurotrophin-1/B cell stimulating factor-3 (NNT-1/BSF-3), is a recently identified member of the interleukin (IL)-6 family of cytokines that share gp130 as a signal-transducing receptor component. In this study, we demonstrate that CLC is expressed in fetal mouse neuroepithelial cells and has a potential to induce their astrocyte differentiation in a synergistic manner with bone-morphogenetic protein (BMP)-2, which is also expressed in the fetal mouse brain. CLC-stimulation led to promoter activation of the gene for an astrocyte marker, glial fibrillary acidic protein (GFAP), which was clearly inhibited by expression of a dominant negative form of a transcription factor, STAT3, or by introduction of a mutation in a single STAT3-binding site in the promoter, suggesting a critical role of STAT3 in the CLC-induced GFAP transcription. These results suggest that CLC plays a role in astrocyte differentiation via STAT3 activation within the developing brain. |
| Links | |
| Keywords |
Animals; Astrocytes/cytology; Astrocytes/metabolism; Binding Sites; Bone Morphogenetic Protein 2; Bone Morphogenetic Proteins/metabolism; Brain/metabolism; Cell Differentiation; Cells, Cultured; Cytokines/metabolism; Cytokines/physiology; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Genes, Dominant; Glial Fibrillary Acidic Protein/metabolism; Immunoblotting; Mice; Mice, Inbred ICR; Microscopy, Fluorescence; Neurons/metabolism; Phosphorylation; Promoter Regions, Genetic; Protein Binding; Reverse Transcriptase Polymerase Chain Reaction; STAT3 Transcription Factor; Time Factors; Tissue Distribution; Trans-Activators/genetics; Trans-Activators/metabolism; Transcription, Genetic; Transforming Growth Factor beta |
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Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
MOUSE:CLCF1 |
involved_in |
GO:0048711: positive regulation of astrocyte differentiation |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
MOUSE:CLCF1 |
involved_in |
GO:0042531: positive regulation of tyrosine phosphorylation of STAT protein |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
See also
References
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