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PMID:12088286

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Citation

Glass, R, Loesch, A, Bodin, P and Burnstock, G (2002) P2X4 and P2X6 receptors associate with VE-cadherin in human endothelial cells. Cell. Mol. Life Sci. 59:870-81

Abstract

We investigated the expression of P2X4 and P2X6 receptors on human umbilical vein endothelial cells (HUVECs) and found that both P2X receptor subtypes on plasma membranes are largely restricted to areas of cell-cell contact. Co-labelling experiments at the confocal and electron microscopy levels revealed that P2X4 and P2X6 receptors are strongly co-localised with the cell adhesion molecule VE-cadherin. The P2X4 and P2X6 receptors on plasma membranes at cellular junctions are rapidly (within 5 min) internalised specifically after decreasing extracellular [Ca2+]. Disruption of microfilaments, microtubules and integrin-mediated adhesion or stimulation of P2 receptors with ATP did not alter P2X4 and P2X6 receptor expression on HUVEC plasma membranes. Membraneous P2X4 and P2X6 receptors resisted extraction with Triton-X 100, whereas cytoplasmic P2X receptors were Triton-X 100 soluble. P2X4 receptors, but not P2X6 receptors, could be co-immunoprecipitated with VE-cadherin and vice versa. We conclude that P2X4 and P2X6 receptors are associated with VE-cadherin at HUVEC adherens junctions.

Links

PubMed

Keywords

Antigens, CD; Cadherins/metabolism; Calcium/metabolism; Cell Fractionation; Cell Line; Cell Membrane/drug effects; Cell Membrane/metabolism; Cells, Cultured; Detergents/pharmacology; Endothelium, Vascular/metabolism; Endothelium, Vascular/ultrastructure; Humans; Immunohistochemistry; Integrins/metabolism; Neuropeptides/metabolism; Octoxynol/pharmacology; Receptors, Purinergic P2/metabolism; Receptors, Purinergic P2X4

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:P2RX6

enables

GO:0005524: ATP binding

ECO:0000303: author statement without traceable support used in manual assertion

F

Seeded From UniProt

complete

HUMAN:P2RX6

located_in

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:P2RX6

located_in

GO:0030054: cell junction

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:P2RX6

enables

GO:0004931: extracellularly ATP-gated cation channel activity

ECO:0000303: author statement without traceable support used in manual assertion

F

Seeded From UniProt

complete

HUMAN:P2RX6

enables

GO:0001614: purinergic nucleotide receptor activity

ECO:0000303: author statement without traceable support used in manual assertion

F

Seeded From UniProt

complete

HUMAN:P2RX4

located_in

GO:0048471: perinuclear region of cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:P2RX4

located_in

GO:0030054: cell junction

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:P2RX4

enables

GO:0045296: cadherin binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P33151

F

Seeded From UniProt

complete

HUMAN:P2RX4

involved_in

GO:0042118: endothelial cell activation

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

HUMAN:CADH5

enables

GO:0005102: signaling receptor binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q99571

F

Seeded From UniProt

complete

Notes

See also

References

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