PMID:12086627

Citation	Proft, M and Struhl, K (2002) Hog1 kinase converts the Sko1-Cyc8-Tup1 repressor complex into an activator that recruits SAGA and SWI/SNF in response to osmotic stress. Mol. Cell 9:1307-17
Abstract	The yeast ATF/CREB repressor Sko1(Acr1) regulates genes that are induced upon hyperosmotic stress by recruiting the Cyc8(Ssn6)-Tup1 corepressor complex to target promoters. During hyperosmotic stress, Hog1 MAP kinase associates with target promoters, phosphorylates Sko1, and converts Sko1 into a transcriptional activator. Unexpectedly, Tup1 remains bound to target promoters during osmotic stress. Sko1, Hog1, and Tup1 are all important for recruitment of SAGA histone acetylase and SWI/SNF nucleosome-remodeling complexes to osmotic-inducible promoters, and both complexes are important for activation upon osmotic stress. Thus, osmotic induction involves a switch of Sko1-Cyc8-Tup1 from a repressing to an activating state in a process that is triggered by Hog1 phosphorylation. Cyc8-Tup1 is not simply a corepressor but is also involved in recruiting SWI/SNF and SAGA during the transcriptional induction process.
Links	PubMed
Keywords	Basic-Leucine Zipper Transcription Factors; DNA-Binding Proteins/metabolism; Fungal Proteins/metabolism; Gene Expression Regulation, Fungal; Mitogen-Activated Protein Kinases/metabolism; Models, Genetic; Nuclear Proteins; Osmolar Concentration; Promoter Regions, Genetic; Repressor Proteins/metabolism; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism; Transcription, Genetic
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
YEAST:SKO1	enables	GO:0000978: RNA polymerase II cis-regulatory region sequence-specific DNA binding	ECO:0000314: direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
YEAST:SKO1	enables	GO:0001228: DNA-binding transcription activator activity, RNA polymerase II-specific	ECO:0000314: direct assay evidence used in manual assertion		F	occurs_at:(SO:0001952)	Seeded From UniProt	complete
YEAST:SKO1	involved_in	GO:0045944: positive regulation of transcription by RNA polymerase II	ECO:0000314: direct assay evidence used in manual assertion		P	part_of:(GO:0071470)	Seeded From UniProt	complete
YEAST:SKO1	enables	GO:0000981: DNA-binding transcription factor activity, RNA polymerase II-specific	ECO:0000315: mutant phenotype evidence used in manual assertion		F		Seeded From UniProt	complete
YEAST:SKO1	enables	GO:0001228: DNA-binding transcription activator activity, RNA polymerase II-specific	ECO:0000315: mutant phenotype evidence used in manual assertion		F	occurs_at:(SO:0001952)	Seeded From UniProt	complete
YEAST:SKO1	involved_in	GO:0045944: positive regulation of transcription by RNA polymerase II	ECO:0000315: mutant phenotype evidence used in manual assertion		P	part_of:(GO:0071470)	Seeded From UniProt	complete
YEAST:SKO1	enables	GO:0051019: mitogen-activated protein kinase binding	ECO:0000315: mutant phenotype evidence used in manual assertion		F		Seeded From UniProt	complete

References

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