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PMID:12084836

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Citation

Broin, M, Cuiné, S, Eymery, F and Rey, P (2002) The plastidic 2-cysteine peroxiredoxin is a target for a thioredoxin involved in the protection of the photosynthetic apparatus against oxidative damage. Plant Cell 14:1417-32

Abstract

The chloroplastic drought-induced stress protein of 32 kD (CDSP32) is composed of two thioredoxin modules and is induced by environmental and oxidative stress conditions. We investigated whether the plastidic protein BAS1, which is related to eubacterial 2-Cys peroxiredoxin, is a target for CDSP32. Using a CDSP32 active-site mutant, we showed that the BAS1 and CDSP32 proteins form a mixed disulfide complex in vitro. Moreover, affinity chromatography indicated that BAS1 is a major target for CDSP32 in chloroplasts. CDSP32 was able to reduce BAS1 in vitro, and BAS1 displayed CDSP32-dependent peroxidase activity. The function of CDSP32 was investigated in transgenic potato lines without detectable levels of the protein as a result of cosuppression. Under conditions of photooxidative stress induced by incubation with either methyl viologen or t-butyl hydroperoxide or by exposure to low temperature under high light, plants lacking CDSP32 exhibited decreased maximal photosystem II photochemical efficiencies compared with the wild type and transgenic controls. In addition, plants without CDSP32 retained much less chlorophyll than controls under stress, indicating increased damage to photosynthetic membranes. We conclude that CDSP32 is a thioredoxin with a critical role in plastid defense against oxidative damage and that this role is related to its function as a physiological electron donor to the BAS1 peroxiredoxin.

Links

PubMed PMC150789

Keywords

Amino Acid Sequence; Arabidopsis/enzymology; Arabidopsis/genetics; Arabidopsis/metabolism; Kinetics; Molecular Sequence Data; Oxidative Stress/drug effects; Peroxidases/chemistry; Peroxidases/genetics; Peroxidases/metabolism; Peroxiredoxins; Photosynthesis/physiology; Plant Proteins/chemistry; Plant Proteins/genetics; Plant Proteins/metabolism; Recombinant Proteins/metabolism; Sequence Alignment; Sequence Homology, Amino Acid; Thioredoxins/pharmacology; Time Factors

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:BAS1A

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q9SGS4

F

Seeded From UniProt

complete

ARATH:BAS1A

enables

GO:0004601: peroxidase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:CDSP

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q96291

F

Seeded From UniProt

complete

ARATH:CDSP

involved_in

GO:0006979: response to oxidative stress

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

ARATH:CDSP

involved_in

GO:0009414: response to water deprivation

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

ARATH:CDSP

involved_in

GO:0045454: cell redox homeostasis

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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