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PMID:12079404

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Citation

Del Signore, A, Gotti, C, De Stefano, ME, Moretti, M and Paggi, P (2002) Dystrophin stabilizes alpha 3- but not alpha 7-containing nicotinic acetylcholine receptor subtypes at the postsynaptic apparatus in the mouse superior cervical ganglion. Neurobiol. Dis. 10:54-66

Abstract

The nicotinic acetylcholine receptor (nAChR) subtypes were characterized in the superior cervical ganglion (SCG) of wild-type and dystrophin-lacking mdx mice. The binding of Epibatidine and alphaBungarotoxin, ligands for alpha3- and alpha7-containing receptors, respectively, revealed, for each ligand, a single class of high-affinity binding sites, with similar affinity in both wild-type and mdx mice. The Epibatidine-labeled receptors were immunoprecipitated by antibodies against the alpha3, beta2, and beta4 subunits. Immunocytochemistry showed that the percentage of alpha3-, beta2-, and beta4- but not of alpha7-immunopositive postsynaptic specializations was significantly lower in mdx than in wild-type mouse SCG. These observations suggest that the mouse SCG contains nAChRs, stabilized by dystrophin, in which the alpha3 subunit is associated with the beta2 and/or beta4 subunits. Conversely, dystrophin is not involved in the stabilization of the alpha7-containing nAChRs, as the percentage of alpha7-immunopositive synapses is similar in both wild-type and mdx mouse SCG.

Links

PubMed

Keywords

Animals; Antibody Specificity; Binding Sites; Bridged Bicyclo Compounds, Heterocyclic/metabolism; Dystrophin/deficiency; Dystrophin/genetics; Humans; Immunohistochemistry; Mice; Mice, Inbred C57BL; Mice, Inbred mdx; Nicotinic Agonists/metabolism; Pyridines/metabolism; Rats; Receptors, Nicotinic/analysis; Receptors, Nicotinic/immunology; Receptors, Nicotinic/metabolism; Superior Cervical Ganglion/chemistry; Superior Cervical Ganglion/metabolism; Superior Cervical Ganglion/ultrastructure; Synapses/chemistry; Synapses/metabolism; Synapses/ultrastructure; Tritium/metabolism; alpha7 Nicotinic Acetylcholine Receptor

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:ACHA7

located_in

GO:0099060: integral component of postsynaptic specialization membrane

ECO:0006003: electron microscopy evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:ACHA7

located_in

GO:0098981: cholinergic synapse

ECO:0006003: electron microscopy evidence used in manual assertion

C

  • part_of:(UBERON:0001989)

Seeded From UniProt

complete

MOUSE:ACHA3

located_in

GO:0099060: integral component of postsynaptic specialization membrane

ECO:0006003: electron microscopy evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:ACHA3

located_in

GO:0098981: cholinergic synapse

ECO:0006003: electron microscopy evidence used in manual assertion

C

  • part_of:(UBERON:0001989)

Seeded From UniProt

complete

MOUSE:ACHB2

located_in

GO:0099060: integral component of postsynaptic specialization membrane

ECO:0006003: electron microscopy evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:ACHB2

located_in

GO:0098981: cholinergic synapse

ECO:0006003: electron microscopy evidence used in manual assertion

C

  • part_of:(UBERON:0001989)

Seeded From UniProt

complete

MOUSE:ACHB2

part_of

GO:0098981: cholinergic synapse

ECO:0006003: electron microscopy evidence used in manual assertion

C

part_of:(UBERON:0001989)

Seeded From UniProt

complete

MOUSE:ACHB2

part_of

GO:0099060: integral component of postsynaptic specialization membrane

ECO:0006003: electron microscopy evidence used in manual assertion

C

  • part_of:(GO:0098981)
  • part_of:(UBERON:0000061)
  • part_of:(UBERON:0001989)

Seeded From UniProt

complete

MOUSE:ACHA7

part_of

GO:0098981: cholinergic synapse

ECO:0006003: electron microscopy evidence used in manual assertion

C

part_of:(UBERON:0001989)

Seeded From UniProt

complete

MOUSE:ACHA7

part_of

GO:0099060: integral component of postsynaptic specialization membrane

ECO:0006003: electron microscopy evidence used in manual assertion

C

  • part_of:(GO:0098981)
  • part_of:(UBERON:0000061)
  • part_of:(UBERON:0001989)

Seeded From UniProt

complete

Notes

See also

References

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