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PMID:12040021

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Citation

Brendel, C, Gelman, L and Auwerx, J (2002) Multiprotein bridging factor-1 (MBF-1) is a cofactor for nuclear receptors that regulate lipid metabolism. Mol. Endocrinol. 16:1367-77

Abstract

Multiprotein bridging factor (MBF-1) is a cofactor that was first described for its capacity to modulate the activity of fushi tarazu factor 1, a nuclear receptor originally implicated in Drosophila development. Recently, it has been shown that human MBF-1 stimulates the transcriptional activity of steroidogenic factor 1, a human homolog of fushi tarazu factor 1, which is implicated in steroidogenesis. Here we show that this cofactor enhances the transcriptional activity of several nonsteroid nuclear receptors that are implicated in lipid metabolism, i.e. the liver receptor homolog 1, the liver X receptor alpha, and PPARgamma. MBF-1 interacts with distinct domains in these receptors, depending on whether the receptor binds DNA as a monomer or as a heterodimer with RXR. MBF-1 does not possess any of the classical histone modifying activities such as histone acetyl- or methyl transferase activities, linked to chromatin remodeling, but interacts in vitro with the transcription factor IID complex. MBF-1 seems therefore to act as a bridging factor enabling interactions of nuclear receptors with the transcription machinery.

Links

PubMed

Keywords

Acetyltransferases/metabolism; Calmodulin-Binding Proteins; Cell Line; DNA-Binding Proteins; Drosophila Proteins; Histone Acetyltransferases; Humans; Lipid Metabolism; Methyltransferases/metabolism; Orphan Nuclear Receptors; RNA, Messenger/genetics; RNA, Messenger/metabolism; Receptors, Cytoplasmic and Nuclear/metabolism; Saccharomyces cerevisiae Proteins/metabolism; Steroidogenic Factor 1; Trans-Activators/genetics; Trans-Activators/metabolism; Transcription Factor TFIID/metabolism; Transcription Factors/metabolism; Transcription, Genetic

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:NR5A2

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:O60869

F

Seeded From UniProt

complete

HUMAN:EDF1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:O00482

F

Seeded From UniProt

complete

HUMAN:EDF1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P37231

F

Seeded From UniProt

complete

HUMAN:EDF1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q13133

F

Seeded From UniProt

complete

HUMAN:EDF1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P20226

F

Seeded From UniProt

complete

HUMAN:EDF1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P19793

F

Seeded From UniProt

complete

HUMAN:EDF1

involved_in

GO:0019216: regulation of lipid metabolic process

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

HUMAN:EDF1

involved_in

GO:0006355: regulation of transcription, DNA-templated

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

HUMAN:EDF1

enables

GO:0001094: TFIID-class transcription factor complex binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:EDF1

enables

GO:0003713: transcription coactivator activity

ECO:0000315: mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:EDF1

involved_in

GO:0045446: endothelial cell differentiation

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

HUMAN:RXRA

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:O60869

F

Seeded From UniProt

complete

HUMAN:TBP

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:O60869

F

Seeded From UniProt

complete

HUMAN:PPARG

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:O60869

F

Seeded From UniProt

complete

HUMAN:NR1H3

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:O60869

F

Seeded From UniProt

complete

HUMAN:EP300

enables

GO:0004402: histone acetyltransferase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

MOUSE:EP300

enables

GO:0004402: histone acetyltransferase activity

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:Q09472

F

Seeded From UniProt

complete


See also

References

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