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PMID:12034757

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Citation

Capiaux, H, Lesterlin, C, Pérals, K, Louarn, JM and Cornet, F (2002) A dual role for the FtsK protein in Escherichia coli chromosome segregation. EMBO Rep. 3:532-6

Abstract

FtsK is a multifunctional protein that acts in Escherichia coli cell division and chromosome segregation. Its C-terminal domain is required for XerCD-mediated recombination between dif sites that resolve chromosome dimers formed by recombination between sister chromosomes. We report the construction and analysis of a set of strains carrying different Xer recombination sites in place of dif, some of which recombine in an FtsK-independent manner. The results show that FtsK-independent Xer recombination does not support chromosome dimer resolution. Furthermore, resolution of dimers by the Cre/loxP system also requires FtsK. These findings reveal a second role for FtsK during chromosome dimer resolution in addition to XerCD activation. We propose that FtsK acts to position the dif regions, thus allowing a productive synapse between dif sites.

Links

PubMed PMC1084150 Online version:10.1093/embo-reports/kvf116

Keywords

Base Sequence; Chromosome Segregation; Chromosomes, Bacterial/physiology; DNA Nucleotidyltransferases/metabolism; Escherichia coli/genetics; Escherichia coli Proteins/physiology; Integrases/metabolism; Membrane Proteins/physiology; Molecular Sequence Data; Rec A Recombinases/metabolism; Recombinases; Sequence Alignment; Viral Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ECOLI:FTSK

involved_in

GO:0007059: chromosome segregation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:FTSK

involved_in

GO:0007059: chromosome segregation

ECO:0000316: genetic interaction evidence used in manual assertion

UniProtKB:P0A734
UniProtKB:P0AEZ3
UniProtKB:P18196

P

Seeded From UniProt

complete

See also

References

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