GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:12029045

From GONUTS
Jump to: navigation, search
Citation

Davis, EO, Dullaghan, EM and Rand, L (2002) Definition of the mycobacterial SOS box and use to identify LexA-regulated genes in Mycobacterium tuberculosis. J. Bacteriol. 184:3287-95

Abstract

The bases of the mycobacterial SOS box important for LexA binding were determined by replacing each base with every other and examining the effect on the induction of a reporter gene following DNA damage. This analysis revealed that the SOS box was longer than originally thought by 2 bp in each half of the palindromic site. A search of the Mycobacterium tuberculosis genome sequence with the new consensus, TCGAAC(N)(4)GTTCGA, identified 4 sites which were perfect matches and 12 sites with a single mismatch which were predicted to bind LexA. Genes which could potentially be regulated by these SOS boxes were ascertained from their positions relative to the sites. Examination of expression data for these genes following DNA damage identified 12 new genes which are most likely regulated by LexA as well as the known M. tuberculosis DNA damage-inducible genes recA, lexA, and ruvC. Of these 12 genes, only 2 have a predicted function: dnaE2, a component of DNA polymerase III, and linB, which is similar to 1,3,4,6-tetrachloro-1,4-cylcohexadiene hydrolase. Curiously, of the remaining 10 genes predicted to be LexA regulated, 7 are members of the M. tuberculosis 13E12 repeat family, which has some of the characteristics of mobile elements.

Links

PubMed PMC135081

Keywords

Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Base Sequence; DNA Damage; Gene Expression Profiling; Gene Expression Regulation, Bacterial; Molecular Sequence Data; Mycobacterium tuberculosis/genetics; Mycobacterium tuberculosis/metabolism; Oligonucleotide Array Sequence Analysis; Rec A Recombinases/genetics; SOS Response (Genetics)/genetics; SOS Response (Genetics)/physiology; Serine Endopeptidases/genetics; Serine Endopeptidases/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MYCS2:LEXA

enables

GO:0000976: transcription cis-regulatory region binding

ECO:0005667: site-directed mutagenesis phenotypic evidence used in manual assertion

F

Seeded From UniProt

complete

MYCS2:LEXA

enables

GO:0001217: DNA-binding transcription repressor activity

ECO:0005667: site-directed mutagenesis phenotypic evidence used in manual assertion

F

Seeded From UniProt

complete

MYCS2:LEXA

part_of

GO:0032993: protein-DNA complex

ECO:0005667: site-directed mutagenesis phenotypic evidence used in manual assertion

C

Seeded From UniProt

complete

MYCTU:LEXA

involved_in

GO:0045892: negative regulation of transcription, DNA-templated

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.