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PMID:11976750

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Citation

Brinkkötter, A, Shakeri-Garakani, A and Lengeler, JW (2002) Two class II D-tagatose-bisphosphate aldolases from enteric bacteria. Arch. Microbiol. 177:410-9

Abstract

Escherichia coli, Salmonella enterica, Klebsiella pneumoniaeand Klebsiella oxytocawere found to contain two D-tagatose 1,6-bisphosphate (TagBP)-specific aldolases involved in catabolism of galactitol (genes gatY gatZ) and of N-acetyl-galactosamine and D-galactosamine (genes kbaY kbaZ,also called agaY agaZ). The two aldolases were closely related (> or = 53.8% identical amino acids) and could substitute for each other in vivo. The catalytic subunits GatY or KbaY alone were sufficient to show aldolase activity. Although substantially shorter than other aldolases (285 amino acids, instead of 358 and 349 amino acids), these subunits contained most or all of the residues that have been identified as essential in substrate/product recognition and catalysis for class II aldolases. In contrast to these, both aldolases required subunits GatZ or KbaZ (420 amino acids) for full activity and for good in vivo and in vitro stability. The Z subunits alone did not show any aldolase activity. Close relatives of these new TagBP aldolases were found in several gram-negative and gram-positive bacteria, e.g., Streptomyces coelicolor.

Links

PubMed Online version:10.1007/s00203-002-0406-6

Keywords

Aldehyde-Lyases/chemistry; Aldehyde-Lyases/classification; Aldehyde-Lyases/genetics; Aldehyde-Lyases/metabolism; Amino Acid Sequence; Binding Sites; Catalysis; Cloning, Molecular; Enterobacteriaceae/enzymology; Enterobacteriaceae/genetics; Escherichia coli/enzymology; Escherichia coli/genetics; Genes, Bacterial/genetics; Hexosediphosphates/metabolism; Kinetics; Klebsiella pneumoniae/enzymology; Klebsiella pneumoniae/genetics; Molecular Sequence Data; Molecular Weight; Sequence Alignment; Sequence Homology, Amino Acid; Substrate Specificity

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ECOLI:ALF

GO:0004332: fructose-bisphosphate aldolase activity

ECO:0000315:

F

Table 2. Aldolase activity is greatly reduced when a mutant strain of E. coli containing a temperature sensitive form of fbaA is incubated at 42 degrees Celsius, thus rendering the gene inactive.

complete

ECOLI:ALF

GO:0006096: glycolysis

ECO:0000315:

P

Table 2. A mutant strain of E. coli containing a temperature sensitive form of fbaA is unable to grow on galactitol when incubated at 42 degrees Celsius(fbaA is inactive at this temperature).

complete

ECOLI:GATY

enables

GO:0009025: tagatose-bisphosphate aldolase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ECOLI:KBAY

enables

GO:0009025: tagatose-bisphosphate aldolase activity

ECO:0000315: mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

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