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PMID:11865031

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Citation

Jiang, FX, Stanley, EG, Gonez, LJ and Harrison, LC (2002) Bone morphogenetic proteins promote development of fetal pancreas epithelial colonies containing insulin-positive cells. J. Cell. Sci. 115:753-60

Abstract

Extracellular signals that guide pancreas cell development are not well characterized. In an in vitro culture system of dissociated pancreas cells from the E15.5 mouse fetus we show that, in the presence of the extracellular matrix protein laminin-1, bone morphogenetic proteins (BMPs-4, -5 and -6) promote the development of cystic epithelial colonies. Transforming growth factor beta1 (TGF-beta1) and activin A antagonise this effect of BMP-6 and inhibit colony formation. Histological analysis revealed that the colonies are composed of E-cadherin-positive epithelial cells, which in localised areas are insulin positive. The colonies also contain occasional glucagon-positive cells, but no somatostatin- or alpha-amylase-positive cells. These findings indicate that members of the TGF-beta superfamily regulate pancreas epithelial cell development and can promote the formation of islet-like structures in vitro.

Links

PubMed

Keywords

Activins/genetics; Activins/physiology; Animals; Bone Morphogenetic Protein 4; Bone Morphogenetic Protein 5; Bone Morphogenetic Protein 6; Bone Morphogenetic Proteins/genetics; Bone Morphogenetic Proteins/metabolism; Bone Morphogenetic Proteins/pharmacology; Cadherins/metabolism; Cell Division; Cells, Cultured; Colony-Forming Units Assay; Embryonic and Fetal Development; Epithelial Cells/chemistry; Epithelial Cells/drug effects; Epithelial Cells/metabolism; Gene Expression Regulation, Developmental; Glucagon/metabolism; Inhibin-beta Subunits/genetics; Inhibin-beta Subunits/physiology; Insulin/analysis; Laminin/metabolism; Mice; Mice, Inbred CBA; Pancreas/cytology; Pancreas/embryology; Pancreas/metabolism; Transforming Growth Factor beta/genetics; Transforming Growth Factor beta/physiology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:BMP6

involved_in

GO:0003323: type B pancreatic cell development

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:BMP6

involved_in

GO:0050679: positive regulation of epithelial cell proliferation

ECO:0000314: direct assay evidence used in manual assertion

P

  • happens_during:(GO:0031016)
  • occurs_in:(CL:0002079)

Seeded From UniProt

complete

HUMAN:BMP5

involved_in

GO:0003323: type B pancreatic cell development

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:BMP5

involved_in

GO:0050679: positive regulation of epithelial cell proliferation

ECO:0000314: direct assay evidence used in manual assertion

P

  • happens_during:(GO:0031016)
  • occurs_in:(CL:0002079)

Seeded From UniProt

complete

HUMAN:BMP4

involved_in

GO:0050679: positive regulation of epithelial cell proliferation

ECO:0000314: direct assay evidence used in manual assertion

P

  • happens_during:(GO:0031016)
  • occurs_in:(CL:0002079)

Seeded From UniProt

complete

HUMAN:BMP4

involved_in

GO:0003323: type B pancreatic cell development

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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