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PMID:11861901

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Citation

Tong, JJ, Liu, J, Bertos, NR and Yang, XJ (2002) Identification of HDAC10, a novel class II human histone deacetylase containing a leucine-rich domain. Nucleic Acids Res. 30:1114-23

Abstract

Histone acetylation is important for regulating chromatin structure and gene expression. Three classes of mammalian histone deacetylases have been identified. Among class II, there are five known members, namely HDAC4, HDAC5, HDAC6, HDAC7 and HDAC9. Here we describe the identification and characterization of a novel class II member termed HDAC10. It is a 669 residue polypeptide with a bipartite modular structure consisting of an N-terminal Hda1p-related putative deacetylase domain and a C-terminal leucine-rich domain. HDAC10 is widely expressed in adult human tissues and cultured mammalian cells. It is enriched in the cytoplasm and this enrichment is not sensitive to leptomycin B, a specific inhibitor known to block the nuclear export of other class II members. The leucine-rich domain of HDAC10 is responsible for its cytoplasmic enrichment. Recombinant HDAC10 protein possesses histone deacetylase activity, which is sensitive to trichostatin A, a specific inhibitor for known class I and class II histone deacetylases. When tethered to a promoter, HDAC10 is able to repress transcription. Furthermore, HDAC10 interacts with HDAC3 but not with HDAC4 or HDAC6. These results indicate that HDAC10 is a novel class II histone deacetylase possessing a unique leucine-rich domain.

Links

PubMed PMC101247

Keywords

Amino Acid Sequence; Animals; Cell Line; Cloning, Molecular; Cytoplasm/metabolism; Genes, Reporter; HeLa Cells; Histone Deacetylases/chemistry; Histone Deacetylases/genetics; Histone Deacetylases/physiology; Humans; Leucine/chemistry; Mice; Molecular Sequence Data; Protein Structure, Tertiary; RNA, Messenger/biosynthesis; Rats; Repressor Proteins/chemistry; Repressor Proteins/genetics; Repressor Proteins/physiology; Sequence Homology, Amino Acid; Tissue Distribution; Transcription, Genetic

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:HDAC3

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q969S8

F

Seeded From UniProt

complete

HUMAN:HDA10

involved_in

GO:0016575: histone deacetylation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:HDA10

enables

GO:0019899: enzyme binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:O15379

F

Seeded From UniProt

complete

HUMAN:HDA10

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:HDA10

located_in

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:HDA10

involved_in

GO:0006355: regulation of transcription, DNA-templated

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:HDA10

involved_in

GO:0006325: chromatin organization

ECO:0000303: author statement without traceable support used in manual assertion

P

Seeded From UniProt

complete

HUMAN:HDA10

part_of

GO:0000118: histone deacetylase complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:HDA10

enables

GO:0004407: histone deacetylase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:HDA10

enables

GO:0042826: histone deacetylase binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:HDA10

involved_in

GO:0045892: negative regulation of transcription, DNA-templated

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:HDAC6

located_in

GO:0005737: cytoplasm

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:Q969S8

C

Seeded From UniProt

complete

HUMAN:HDAC6

part_of

GO:0005737: cytoplasm

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:Q969S8

C

Seeded From UniProt

complete

HUMAN:HDAC6

involved_in

GO:0016575: histone deacetylation

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:Q969S8

P

Seeded From UniProt

complete

HUMAN:HDAC6

enables

GO:0019899: enzyme binding

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:Q969S8

F

Seeded From UniProt

complete

HUMAN:HDAC6

involved_in

GO:0045892: negative regulation of transcription, DNA-templated

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:Q969S8

P

Seeded From UniProt

complete


See also

References

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