GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:11854298

From GONUTS
Jump to: navigation, search
Citation

Kressler, D, Schreiber, SN, Knutti, D and Kralli, A (2002) The PGC-1-related protein PERC is a selective coactivator of estrogen receptor alpha. J. Biol. Chem. 277:13918-25

Abstract

Peroxisome proliferator-activated receptor gamma coactivator-1 (PGC-1) is a tissue-specific coactivator that enhances the activity of many nuclear receptors and coordinates transcriptional programs important for energy metabolism. We describe here a novel PGC-1-related coactivator that is expressed in a similar tissue-specific manner as PGC-1, with the highest levels in heart and skeletal muscle. In contrast to PGC-1, the new coactivator shows high receptor specificity. It enhances potently the activity of estrogen receptor (ER) alpha, while having only small effects on other receptors. Because of its nuclear receptor selectivity, we have termed the new protein PERC (PGC-1 related Estrogen Receptor Coactivator). We show here that the coactivation function of PERC relies on a bipartite transcriptional activation domain and two LXXLL motifs that interact with the AF2 domain of ERalpha in an estrogen-dependent manner. PERC and PGC-1 are likely to have different functions in ER signaling. Whereas PERC acts selectively on ERalpha and not on the second estrogen receptor ERbeta, PGC-1 coactivates strongly both ERs. Moreover, PERC and PGC-1 show distinct preferences for enhancing ERalpha in different promoter contexts. Finally, PERC enhances the ERalpha-mediated response to the partial agonist tamoxifen, while PGC-1 modestly represses it. The two coactivators are likely to mediate distinct, tissue-specific responses to estrogens.

Links

PubMed Online version:10.1074/jbc.M201134200

Keywords

Amino Acid Motifs; Amino Acid Sequence; Animals; COS Cells; Carrier Proteins/chemistry; Carrier Proteins/metabolism; Cell Nucleus/metabolism; Cloning, Molecular; Estrogen Receptor alpha; HeLa Cells; Humans; Ligands; Microscopy, Fluorescence; Molecular Sequence Data; Muscle, Skeletal/metabolism; Myocardium/metabolism; Plasmids/metabolism; Promoter Regions, Genetic; Protein Binding; Protein Structure, Tertiary; RNA/metabolism; RNA, Messenger/metabolism; Receptors, Estrogen/metabolism; Sequence Homology, Amino Acid; Tissue Distribution; Transcription Factors/chemistry; Transcription Factors/metabolism; Transcriptional Activation; Transfection; Tumor Cells, Cultured; Two-Hybrid System Techniques

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ZOAVI:Q800Q2

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q86YN6

F

Seeded From UniProt

complete

HUMAN:PRGC2

involved_in

GO:0030520: intracellular estrogen receptor signaling pathway

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:PRGC2

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:PRGC2

enables

GO:0030331: estrogen receptor binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:PRGC2

enables

GO:0030374: nuclear receptor coactivator activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:PRGC2

part_of

GO:0016592: mediator complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:PRGC2

enables

GO:0003712: transcription coregulator activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:PRGC2

enables

GO:0003723: RNA binding

ECO:0000303: author statement without traceable support used in manual assertion

F

Seeded From UniProt

complete

HUMAN:PRGC2

involved_in

GO:0045944: positive regulation of transcription by RNA polymerase II

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:PRGC2

enables

GO:0050682: AF-2 domain binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q800Q2

F

Seeded From UniProt

complete

HUMAN:PRGC1

involved_in

GO:0001678: cellular glucose homeostasis

ECO:0000303: author statement without traceable support used in manual assertion

P

Seeded From UniProt

complete

HUMAN:PRGC1

involved_in

GO:0006094: gluconeogenesis

ECO:0000303: author statement without traceable support used in manual assertion

P

Seeded From UniProt

complete

HUMAN:PRGC1

involved_in

GO:0007005: mitochondrion organization

ECO:0000303: author statement without traceable support used in manual assertion

P

Seeded From UniProt

complete

HUMAN:PRGC1

involved_in

GO:0019395: fatty acid oxidation

ECO:0000303: author statement without traceable support used in manual assertion

P

Seeded From UniProt

complete

HUMAN:PRGC1

involved_in

GO:0042594: response to starvation

ECO:0000303: author statement without traceable support used in manual assertion

P

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.