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PMID:11827972

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Citation

Suzuki, T, Nakamoto, T, Ogawa, S, Seo, S, Matsumura, T, Tachibana, K, Morimoto, C and Hirai, H (2002) MICAL, a novel CasL interacting molecule, associates with vimentin. J. Biol. Chem. 277:14933-41

Abstract

CasL/HEF1 belongs to the p130(Cas) family. It is tyrosine-phosphorylated following beta(1) integrin and/or T cell receptor stimulation and is thus considered to be important for immunological reactions. CasL has several structural motifs such as an SH3 domain and a substrate domain and interacts with many molecules through these motifs. To obtain more insights on the CasL-mediated signal transduction, we sought proteins that interact with the CasL SH3 domain by far Western screening, and we identified a novel human molecule, MICAL (a Molecule Interacting with CasL). MICAL is a protein of 118 kDa and is expressed in the thymus, lung, spleen, kidney, testis, and hematopoietic cells. MICAL has a calponin homology domain, a LIM domain, a putative leucine zipper motif, and a proline-rich PPKPP sequence. MICAL associates with CasL through this PPKPP sequence. MICAL is a cytoplasmic protein and colocalizes with CasL at the perinuclear area. Through the COOH-terminal region, MICAL also associates with vimentin that is a major component of intermediate filaments. Immunostaining revealed that MICAL localizes along with vimentin intermediate filaments. These results suggest that MICAL may be a cytoskeletal regulator that connects CasL to intermediate filaments.

Links

PubMed Online version:10.1074/jbc.M111842200

Keywords

Adaptor Proteins, Signal Transducing; Amino Acid Sequence; Base Sequence; Cell Line; Cytoskeletal Proteins/chemistry; Cytoskeletal Proteins/genetics; Cytoskeletal Proteins/metabolism; DNA, Complementary; Intracellular Signaling Peptides and Proteins; LIM Domain Proteins; Microscopy, Fluorescence; Molecular Sequence Data; Plasmids; Protein Binding; RNA, Messenger/genetics; Vimentin/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:VIME

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q8TDZ2

F

Seeded From UniProt

complete

HUMAN:CASL

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q8TDZ2

F

Seeded From UniProt

complete

HUMAN:MICA1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P08670

F

Seeded From UniProt

complete

HUMAN:MICA1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q14511

F

Seeded From UniProt

complete

HUMAN:MICA1

located_in

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:MICA1

involved_in

GO:0007165: signal transduction

ECO:0000303: author statement without traceable support used in manual assertion

P

Seeded From UniProt

complete

HUMAN:MICA1

involved_in

GO:0007010: cytoskeleton organization

ECO:0000303: author statement without traceable support used in manual assertion

P

Seeded From UniProt

complete

HUMAN:MICA1

located_in

GO:0005882: intermediate filament

ECO:0000303: author statement without traceable support used in manual assertion

C

Seeded From UniProt

complete

HUMAN:MICA1

enables

GO:0017124: SH3 domain binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q14511

F

Seeded From UniProt

complete

HUMAN:CASL

involved_in

GO:0051017: actin filament bundle assembly

ECO:0000303: author statement without traceable support used in manual assertion

P

Seeded From UniProt

complete

HUMAN:CASL

involved_in

GO:0007229: integrin-mediated signaling pathway

ECO:0000303: author statement without traceable support used in manual assertion

P

Seeded From UniProt

complete

HUMAN:CASL

involved_in

GO:0007010: cytoskeleton organization

ECO:0000303: author statement without traceable support used in manual assertion

P

Seeded From UniProt

complete


See also

References

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