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PMID:11809883

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Citation

Plotz, G, Raedle, J, Brieger, A, Trojan, J and Zeuzem, S (2002) hMutSalpha forms an ATP-dependent complex with hMutLalpha and hMutLbeta on DNA. Nucleic Acids Res. 30:711-8

Abstract

The DNA binding properties of hMutSalpha and hMutLalpha and complex formation of hMutSalpha with hMutLalpha and hMutLbeta were investigated using binding experiments on magnetic bead-coupled DNA substrates with nuclear extracts as well as purified proteins. hMutSalpha binding to homoduplex DNA was disrupted by lower NaCl concentrations than hMutSalpha binding to a mismatch. ATP markedly reduced the salt resistance of hMutSalpha binding but hMutSalpha still retained affinity for heteroduplexes. hMutSalpha formed a complex with hMutLalpha and hMutLbeta on DNA in the presence of ATP. This complex only formed on 81mer and not 32mer DNA substrates. Complex formation was enhanced by a mismatch in the DNA substrate, and hMutLalpha and hMutLbeta were shown to enter the complex at different ATP concentrations. Purified hMutLalpha showed an intrinsic affinity for DNA, with a preference for single-stranded over double-stranded DNA.

Links

PubMed PMC100294

Keywords

Adaptor Proteins, Signal Transducing; Adenosine Triphosphatases/metabolism; Adenosine Triphosphate/metabolism; Base Pair Mismatch; Blotting, Western; Carrier Proteins; DNA/genetics; DNA/metabolism; DNA Repair Enzymes; DNA-Binding Proteins/metabolism; Dimerization; HeLa Cells; Humans; Macromolecular Substances; Magnetics; Microspheres; MutS Homolog 2 Protein; Neoplasm Proteins/metabolism; Nuclear Proteins; Osmolar Concentration; Protein Binding; Protein Structure, Quaternary; Protein Subunits; Proto-Oncogene Proteins/metabolism; Thermodynamics

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:MLH1

located_in

GO:0005634: nucleus

ECO:0000305: curator inference used in manual assertion

GO:0003697

C

Seeded From UniProt

complete

HUMAN:PMS2

contributes_to

GO:0003697: single-stranded DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:MSH2

contributes_to

GO:0003690: double-stranded DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:MSH2

contributes_to

GO:0003697: single-stranded DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:MSH2

contributes_to

GO:0032137: guanine/thymine mispair binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:MSH6

contributes_to

GO:0032137: guanine/thymine mispair binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:MSH6

contributes_to

GO:0003690: double-stranded DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:MSH3

NOT|enables

GO:0032137: guanine/thymine mispair binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:MSH3

contributes_to

GO:0003697: single-stranded DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:MLH1

part_of

GO:0005634: nucleus

ECO:0000305: curator inference used in manual assertion

GO:0003697

C

Seeded From UniProt

complete

HUMAN:MLH1

contributes_to

GO:0003697: single-stranded DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

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