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PMID:11781569

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Citation

Vasioukhin, V, Bowers, E, Bauer, C, Degenstein, L and Fuchs, E (2001) Desmoplakin is essential in epidermal sheet formation. Nat. Cell Biol. 3:1076-85

Abstract

We have generated an epidermis-specific desmoplakin (DP) mouse knockout, and show that epidermal integrity requires DP; mechanical stresses to DP-null skin cause intercellular separations. The number of epidermal desmosomes in DP-null skin is similar to wild type (WT), but they lack keratin filaments, which compromise their function. DP-null keratinocytes have few desmosomes in vitro, and are unable to undergo actin reorganization and membrane sealing during epithelial sheet formation. Adherens junctions are also reduced. In vitro, DP transgene expression rescues these defects. DP is therefore required for assembly of functional desmosomes, maintaining cytoskeletal architecture and reinforcing membrane attachments essential for stable intercellular adhesion.

Links

PubMed Online version:10.1038/ncb1201-1076

Keywords

Adherens Junctions/pathology; Adherens Junctions/ultrastructure; Animals; Basement Membrane/chemistry; Cell Adhesion/physiology; Cells, Cultured; Cytoskeletal Proteins/analysis; Cytoskeletal Proteins/genetics; Cytoskeletal Proteins/metabolism; Cytoskeleton/chemistry; Cytoskeleton/pathology; Desmoplakins; Desmosomes/pathology; Desmosomes/ultrastructure; Epidermis/abnormalities; Epidermis/pathology; Gene Expression Regulation, Developmental; Keratinocytes/pathology; Keratinocytes/physiology; Keratins/metabolism; Mice; Mice, Knockout; Microscopy, Electron; Phenotype; Stress, Mechanical

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:DESP

acts_upstream_of_or_within

GO:0034332: adherens junction organization

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:1926500,MGI:MGI:2156542

P

Seeded From UniProt

complete

MOUSE:DESP

acts_upstream_of_or_within

GO:0043588: skin development

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:1926500,MGI:MGI:2156542

P

Seeded From UniProt

complete

MOUSE:DESP

acts_upstream_of_or_within

GO:0098609: cell-cell adhesion

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:1926500,MGI:MGI:2156542

P

Seeded From UniProt

complete


See also

References

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