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PMID:11743113

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Citation

Gocal, GF, Sheldon, CC, Gubler, F, Moritz, T, Bagnall, DJ, MacMillan, CP, Li, SF, Parish, RW, Dennis, ES, Weigel, D and King, RW (2001) GAMYB-like genes, flowering, and gibberellin signaling in Arabidopsis. Plant Physiol. 127:1682-93

Abstract

We have identified three Arabidopsis genes with GAMYB-like activity, AtMYB33, AtMYB65, and AtMYB101, which can substitute for barley (Hordeum vulgare) GAMYB in transactivating the barley alpha-amylase promoter. We have investigated the relationships between gibberellins (GAs), these GAMYB-like genes, and petiole elongation and flowering of Arabidopsis. Within 1 to 2 d of transferring plants from short- to long-day photoperiods, growth rate and erectness of petioles increased, and there were morphological changes at the shoot apex associated with the transition to flowering. These responses were accompanied by accumulation of GAs in the petioles (GA(1) by 11-fold and GA(4) by 3-fold), and an increase in expression of AtMYB33 at the shoot apex. Inhibition of GA biosynthesis using paclobutrazol blocked the petiole elongation induced by long days. Causality was suggested by the finding that, with GA treatment, plants flowered in short days, AtMYB33 expression increased at the shoot apex, and the petioles elongated and grew erect. That AtMYB33 may mediate a GA signaling role in flowering was supported by its ability to bind to a specific 8-bp sequence in the promoter of the floral meristem-identity gene, LEAFY, this same sequence being important in the GA response of the LEAFY promoter. One or more of these AtMYB genes may also play a role in the root tip during germination and, later, in stem tissue. These findings extend our earlier studies of GA signaling in the Gramineae to include a dicot species, Arabidopsis, and indicate that GAMYB-like genes may mediate GA signaling in growth and flowering responses.

Links

PubMed PMC133573

Keywords

Arabidopsis/genetics; Arabidopsis/growth & development; Arabidopsis/metabolism; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Cloning, Molecular; Gene Expression Regulation, Plant; Gibberellins/metabolism; Photoperiod; Plant Root Cap/genetics; Plant Root Cap/metabolism; Plant Stems/genetics; Plant Stems/growth & development; Plant Stems/metabolism; Promoter Regions, Genetic; Seeds/genetics; Seeds/growth & development; Seeds/metabolism; Sequence Homology, Amino Acid; Signal Transduction; alpha-Amylases/genetics; alpha-Amylases/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:MB101

located_in

GO:0005634: nucleus

ECO:0000304: author statement supported by traceable reference used in manual assertion

C

Seeded From UniProt

complete

ARATH:MB101

acts_upstream_of_or_within

GO:0009740: gibberellic acid mediated signaling pathway

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

ARATH:MB101

enables

GO:0003700: DNA-binding transcription factor activity

ECO:0000304: author statement supported by traceable reference used in manual assertion

F

Seeded From UniProt

complete

ARATH:MYB33

involved_in

GO:0009739: response to gibberellin

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:MYB33

located_in

GO:0005634: nucleus

ECO:0000304: author statement supported by traceable reference used in manual assertion

C

Seeded From UniProt

complete

ARATH:MYB33

acts_upstream_of_or_within

GO:0009740: gibberellic acid mediated signaling pathway

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

ARATH:MYB33

enables

GO:0003700: DNA-binding transcription factor activity

ECO:0000304: author statement supported by traceable reference used in manual assertion

F

Seeded From UniProt

complete

ARATH:MYB65

located_in

GO:0005634: nucleus

ECO:0000304: author statement supported by traceable reference used in manual assertion

C

Seeded From UniProt

complete

ARATH:MYB65

acts_upstream_of_or_within

GO:0009740: gibberellic acid mediated signaling pathway

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

ARATH:MYB65

enables

GO:0003700: DNA-binding transcription factor activity

ECO:0000304: author statement supported by traceable reference used in manual assertion

F

Seeded From UniProt

complete


See also

References

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