PMID:11735100

Citation	Moura, MA, Kawashita, NH, Brito, SM, Brito, MN, Kettelhut, IC and Migliorini, RH (2001) Effect of cold acclimation on brown adipose tissue fatty acid synthesis in rats adapted to a high-protein, carbohydrate-free diet. Metab. Clin. Exp. 50:1493-8
Abstract	The effect of cold acclimation on brown adipose tissue (BAT) fatty acid synthesis was investigated in rats adapted to a high-protein, carbohydrate-free diet. At an ambient temperature (25 degrees C), rates of fatty acid synthesis in BAT from rats adapted to the high-protein diet were reduced to 27% of rats fed the balanced diet and increased markedly after cold acclimation (10 days at 4 degrees C), although the increase was smaller than in control rats. BAT weight increase induced by cold acclimation was smaller in rats fed the high-protein diet (30%) than in controls (100%). When expressed per whole tissue, maximal activities of BAT glucose-6-phosphate dehydrogenase, malic enzyme, adenosine triphosphate (ATP)-citrate lyase, and acetyl-coenzyme A carboxylase were markedly reduced in high-protein diet-adapted rats at 25 degrees C and increased after cold acclimation in BAT from the 2 groups. However, when expressed per milligram protein, only acetyl-coenzyme A carboxylase showed an increase in both controls and in rats fed the high-protein diet. G6P-dehydrogenase, malic enzyme, and ATP-citrate lyase increased (per milligram protein) only in rats adapted to the high-protein diet and actually decreased in BAT from cold-acclimated control rats. Initial (before activation) pyruvate dehydrogenase (PDH) complex activity was lower in BAT from rats fed the high-protein diet at 25 degrees C and increased in cold-acclimated rats from the 2 groups. Circulating levels of insulin decreased in the 2 groups after cold acclimation. The data suggest that the cold acclimation-induced increase in BAT lipogenesis in rats adapted to the high-protein diet was due to a restoration of sympathetic activity, which induced both BAT hyperplasia and activation of adipocyte free fatty acid (FFA) synthesis, with an important participation of acetyl-coenzyme A carboxylase and pyruvate dehydrogenase.
Links	PubMed Online version:10.1053/meta.2001.27197
Keywords	ATP Citrate (pro-S)-Lyase/metabolism; Acclimatization; Acetyl-CoA Carboxylase/metabolism; Adaptation, Physiological; Adipose Tissue, Brown/anatomy & histology; Adipose Tissue, Brown/metabolism; Animals; Body Temperature; Cold Temperature; Dietary Carbohydrates/administration & dosage; Dietary Proteins/administration & dosage; Fatty Acids/biosynthesis; Glucosephosphate Dehydrogenase/metabolism; Malate Dehydrogenase/metabolism; Male; Organ Size; Pyruvate Dehydrogenase Complex/metabolism; Rats; Rats, Wistar
edit table

Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
RAT:ACLY	enables	GO:0003878: ATP citrate synthase activity	ECO:0000314: direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
RAT:ACLY	involved_in	GO:0006633: fatty acid biosynthetic process	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
RAT:G6PD	enables	GO:0004345: glucose-6-phosphate dehydrogenase activity	ECO:0000314: direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
RAT:ACACA	enables	GO:0003989: acetyl-CoA carboxylase activity	ECO:0000314: direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
RAT:ACACA	involved_in	GO:0006633: fatty acid biosynthetic process	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
RAT:MAOX	enables	GO:0004470: malic enzyme activity	ECO:0000314: direct assay evidence used in manual assertion		F		Seeded From UniProt	complete

References

See Help:References for how to manage references in GONUTS.