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PMID:11724907

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Citation

Miller, LA, Wert, SE and Whitsett, JA (2001) Immunolocalization of sonic hedgehog (Shh) in developing mouse lung. J. Histochem. Cytochem. 49:1593-604

Abstract

Expression of sonic hedgehog (Shh) is required for normal development of the lung during embryogenesis. Loss of Shh expression in mice results in tracheoesophageal fistula, lung hypoplasia, and abnormal lung lobulation. To determine whether Shh may play a role later in lung morphogenesis, immunostaining for Shh was performed in mouse lung from embryonic day (E) 10.5 to postnatal day (PD) 24. Shh was detected in the distal epithelium of the developing mouse lung from E10.5 to E16.5. From E16.5 until PD15, Shh was present in epithelial cells in both the peripheral and conducting airways. Although all cells of the developing epithelium uniformly expressed Shh at E10.5, Shh expression was restricted to subsets of epithelial cells by E16.5. Between E16.5 and PD15, non-uniform Shh staining of epithelial cells was observed in the conducting airways in a pattern consistent with the distribution of non-ciliated bronchiolar cells (i.e., Clara cells) and the Clara cell marker CCSP. Shh did not co-localize with hepatocyte nuclear factor/forkhead homologue-4 (HFH-4), beta-tubulin, or with the presence of cilia. These results support the concept that Shh plays a distinct regulatory role in the lung later in morphogenesis, when it may influence formation or cytodifferentiation of the conducting airways.

Links

PubMed

Keywords

Animals; Animals, Newborn; DNA-Binding Proteins; Forkhead Transcription Factors; Hedgehog Proteins; Immunohistochemistry; Lung/embryology; Lung/growth & development; Lung/metabolism; Mice; Proteins/metabolism; Respiratory Mucosa/embryology; Respiratory Mucosa/growth & development; Respiratory Mucosa/metabolism; Trans-Activators/metabolism; Tubulin/metabolism; Uteroglobin

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:NKX21

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(EMAPA:16731)

Seeded From UniProt

complete

MOUSE:FOXJ1

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(EMAPA:17655)

Seeded From UniProt

complete

MOUSE:TBB4A

located_in

GO:0005929: cilium

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(EMAPA:17655)

Seeded From UniProt

complete

MOUSE:FOXJ1

part_of

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

part_of:(EMAPA:17655)

Seeded From UniProt

complete

MOUSE:NKX21

part_of

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

part_of:(EMAPA:16731)

Seeded From UniProt

complete


See also

References

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