GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:11715016

From GONUTS
Jump to: navigation, search
Citation

Alcasabas, AA, Osborn, AJ, Bachant, J, Hu, F, Werler, PJ, Bousset, K, Furuya, K, Diffley, JF, Carr, AM and Elledge, SJ (2001) Mrc1 transduces signals of DNA replication stress to activate Rad53. Nat. Cell Biol. 3:958-65

Abstract

Cells experiencing DNA replication stress activate a response pathway that delays entry into mitosis and promotes DNA repair and completion of DNA replication. The protein kinases ScRad53 and SpCds1 (in baker's and fission yeast, respectively) are central to this pathway. We describe a conserved protein Mrc1, mediator of the replication checkpoint, required for activation of ScRad53 and SpCds1 during replication stress. mrc1 mutants are sensitive to hydroxyurea and have a checkpoint defect similar to rad53 and cds1 mutants. Mrc1 may be the replicative counterpart of Rad9 and Crb2, which are required for activating ScRad53 and Chk1 in response to DNA damage.

Links

PubMed Online version:10.1038/ncb1101-958

Keywords

Amino Acid Sequence; Animals; Cell Cycle Proteins/metabolism; DNA Replication; DNA, Fungal/biosynthesis; Enzyme Activation; Fungal Proteins/genetics; Fungal Proteins/metabolism; Genes, Fungal; Humans; Intracellular Signaling Peptides and Proteins; Molecular Sequence Data; Protein Kinases/metabolism; Protein-Serine-Threonine Kinases/metabolism; S Phase; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism; Schizosaccharomyces; Signal Transduction

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:MRC1

involved_in

GO:0000076: DNA replication checkpoint signaling

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:MRC1

involved_in

GO:0031573: mitotic intra-S DNA damage checkpoint signaling

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

SCHPO:MRC1

involved_in

GO:0033314: mitotic DNA replication checkpoint signaling

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.