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PMID:11713579

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Citation

Guerra, B, Boldyreff, B and Issinger, OG (2001) FAS-associated factor 1 interacts with protein kinase CK2 in vivo upon apoptosis induction. Int. J. Oncol. 19:1117-26

Abstract

We show here that in several different cell lines protein kinase CK2 and Fas-associated factor 1 (FAF1) exist together in a complex which is stable to high monovalent salt concentration. The CK2/FAF1 complex formation is significantly increased after induction of apoptosis with various DNA damaging agents. Interestingly this effect is only seen in cell lines with an embryonic origin and not when cells have entered a differentiated state. It is further shown that the CK2 specific phosphorylation sites in the FAF1 molecule, i.e. serines 289 and 291 influence this complex formation. Mutation of the CK2 phosphorylation sites in the FAF1 molecule to alanine leads to a 1.5 to 2.0-fold higher association between CK2 and FAF1. Since the CK2 activity did not increase concomitantly with the complex formation we conclude that the FAF1 becomes to the CK2 enzyme so that a normal enzyme catalysis does not take place anymore. Subcellular localization experiments involving CK2 subunits and FAF1 show a co-localization of both CK2 subunits and FAF1 in the peri-nuclear cytoplasm. The majority of CK2 subunits is found in the nucleus. FAF1 is also found in the nucleoli. The results obtained further support the view that protein kinase CK2 plays an important role in certain steps of apoptosis.

Links

PubMed

Keywords

Adaptor Proteins, Signal Transducing; Animals; Antineoplastic Agents/pharmacology; Apoptosis/physiology; Carrier Proteins/metabolism; Casein Kinase II; Cell Differentiation; Cisplatin/pharmacology; DNA Primers/chemistry; DNA, Complementary; Electrophoresis, Polyacrylamide Gel; Fibroblast Growth Factor 2/metabolism; Gene Expression; Humans; Mice; Mutation; Phosphorylation; Plasmids; Protein-Serine-Threonine Kinases/metabolism; Transfection; Tumor Cells, Cultured/drug effects; Tumor Cells, Cultured/metabolism; Tumor Cells, Cultured/pathology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:FAF1

located_in

GO:0048471: perinuclear region of cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:FAF1

enables

GO:0019901: protein kinase binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:FAF1

enables

GO:0019887: protein kinase regulator activity

ECO:0000303: author statement without traceable support used in manual assertion

F

Seeded From UniProt

complete

HUMAN:FAF1

part_of

GO:0048471: perinuclear region of cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

Notes

See also

References

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