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PMID:11703928

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Citation

Lekven, AC, Thorpe, CJ, Waxman, JS and Moon, RT (2001) Zebrafish wnt8 encodes two wnt8 proteins on a bicistronic transcript and is required for mesoderm and neurectoderm patterning. Dev. Cell 1:103-14

Abstract

In vertebrates, wnt8 has been implicated in the early patterning of the mesoderm. To determine directly the embryonic requirements for wnt8, we generated a chromosomal deficiency in zebrafish that removes the bicistronic wnt8 locus. We report that homozygous mutants exhibit pronounced defects in dorso-ventral mesoderm patterning and in the antero-posterior neural pattern. Despite differences in their signaling activities, either coding region of the bicistronic RNA can rescue the deficiency phenotype. Specific interference of wnt8 translation by morpholino antisense oligomers phenocopies the deficiency, and interference with wnt8 translation in ntl and spt mutants produces embryos lacking trunk and tail. These data demonstrate that the zebrafish wnt8 locus is required during gastrulation to pattern both the mesoderm and the neural ectoderm properly.

Links

PubMed

Keywords

Amino Acid Sequence; Animals; Body Patterning/physiology; Cytoskeletal Proteins; Ectoderm/physiology; Gene Expression Regulation, Developmental; Head/embryology; Mesoderm/physiology; Molecular Sequence Data; Mutagenesis/physiology; Oligonucleotides, Antisense; Open Reading Frames; Phenotype; Proteins/genetics; RNA, Messenger/genetics; T-Box Domain Proteins/genetics; Tail/embryology; Transcription, Genetic/physiology; Wnt Proteins; Zebrafish/embryology; Zebrafish Proteins/genetics

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DANRE:WNT8A

involved_in

GO:0007498: mesoderm development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-041217-12

P

Seeded From UniProt

complete

DANRE:WNT8A

involved_in

GO:0007398: ectoderm development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-041217-12

P

Seeded From UniProt

complete

DANRE:WNT8A

involved_in

GO:0009952: anterior/posterior pattern specification

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-041217-12

P

Seeded From UniProt

complete


See also

References

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