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PMID:11696438

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Citation

Pfander, D, Swoboda, B and Kirsch, T (2001) Expression of early and late differentiation markers (proliferating cell nuclear antigen, syndecan-3, annexin VI, and alkaline phosphatase) by human osteoarthritic chondrocytes. Am. J. Pathol. 159:1777-83

Abstract

Although osteoarthritis is characterized by a progressive loss of the extracellular cartilage matrix, very little is known about the fate of articular chondrocytes during the progression of the disease. In this study we examined the expression of syndecan-3, a marker of early chondrocyte differentiation, and annexin VI, a marker of late chondrocyte differentiation, in mammalian embryonic growth plate cartilage and normal and osteoarthritic human articular cartilage. Whereas syndecan-3 was expressed in the proliferative and hypertrophic zones of growth platecartilage, immunostaining for annexin VI waspredominately found in the hypertrophic and mineralizing zones of fetal bovine growth plate cartilage. Approximately 20% of chondrocytes were immunopositive for syndecan-3 in normal human articular cartilage, the number of syndecan-3-expressing chondrocytes significantly increased during the progression of osteoarthritis with more than 80% syndecan-3-positive cells in the upper zone of severely affected osteoarthritic cartilage. Similarly, the number of annexin VI-expressing cells significantly increased in the upper cartilage zones during the progression of osteoarthritis. Furthermore, immunostaining for proliferating cell nuclear antigen, a marker for cell proliferation, was detected in chondrocytes in the upper zone of osteoarthritic cartilage. Double-labeling experiments with antibodies against syndecan-3 and annexin VI revealed chondrocytes that expressed only syndecan-3, and cells that expressed both syndecan-3 and annexin VI. These results suggest that the expression of early (proliferating cell nuclear antigen, syndecan-3) and late differentiation markers (annexin VI, alkaline phosphatase) is activated in chondrocytes of osteoarthritic cartilage.

Links

PubMed PMC1867060 Online version:10.1016/S0002-9440(10)63024-6

Keywords

Alkaline Phosphatase/metabolism; Animals; Annexin A6/metabolism; Cartilage/cytology; Cartilage/metabolism; Cartilage, Articular/metabolism; Cartilage, Articular/pathology; Cattle/embryology; Chick Embryo; Chondrocytes/metabolism; Growth Plate/cytology; Growth Plate/metabolism; Humans; Membrane Glycoproteins/metabolism; Osteoarthritis/metabolism; Osteoarthritis/pathology; Proliferating Cell Nuclear Antigen/metabolism; Proteoglycans/metabolism; Reference Values; Syndecan-3

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

BOVIN:F1MP76

involved_in

GO:0003419: growth plate cartilage chondrocyte proliferation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

BOVIN:F1MP76

involved_in

GO:0003418: growth plate cartilage chondrocyte differentiation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:ANXA5

located_in

GO:0031982: vesicle

ECO:0000304: author statement supported by traceable reference used in manual assertion

C

Seeded From UniProt

complete

CHICK:ANXA2

located_in

GO:0031982: vesicle

ECO:0000304: author statement supported by traceable reference used in manual assertion

C

Seeded From UniProt

complete

CHICK:ANXA6

located_in

GO:0031982: vesicle

ECO:0000304: author statement supported by traceable reference used in manual assertion

C

Seeded From UniProt

complete

BOVIN:ANXA6

involved_in

GO:0003418: growth plate cartilage chondrocyte differentiation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:ANXA2

involved_in

GO:0055074: calcium ion homeostasis

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

CHICK:ANXA2

part_of

GO:0034704: calcium channel complex

ECO:0000304: author statement supported by traceable reference used in manual assertion

C

Seeded From UniProt

complete

CHICK:ANXA2

enables

GO:0005262: calcium channel activity

ECO:0000304: author statement supported by traceable reference used in manual assertion

F

Seeded From UniProt

complete

CHICK:ANXA2

part_of

GO:0031982: vesicle

ECO:0000304: author statement supported by traceable reference used in manual assertion


C

Seeded From UniProt

complete

CHICK:ANXA5

enables

GO:0005262: calcium channel activity

ECO:0000304: author statement supported by traceable reference used in manual assertion

F

Seeded From UniProt

complete

CHICK:ANXA5

part_of

GO:0031982: vesicle

ECO:0000304: author statement supported by traceable reference used in manual assertion

C

Seeded From UniProt

complete

CHICK:ANXA5

involved_in

GO:0055074: calcium ion homeostasis

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

CHICK:ANXA5

part_of

GO:0034704: calcium channel complex

ECO:0000304: author statement supported by traceable reference used in manual assertion

C

Seeded From UniProt

complete

CHICK:ANXA6

involved_in

GO:0055074: calcium ion homeostasis

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

CHICK:ANXA6

part_of

GO:0031982: vesicle

ECO:0000304: author statement supported by traceable reference used in manual assertion

C

Seeded From UniProt

complete

CHICK:ANXA6

enables

GO:0005262: calcium channel activity

ECO:0000304: author statement supported by traceable reference used in manual assertion

F

Seeded From UniProt

complete

CHICK:ANXA6

part_of

GO:0034704: calcium channel complex

ECO:0000304: author statement supported by traceable reference used in manual assertion

C

Seeded From UniProt

complete

CHICK:SDC3

enables

GO:0019838: growth factor binding

ECO:0000304: author statement supported by traceable reference used in manual assertion

F

Seeded From UniProt

complete

CHICK:SDC3

involved_in

GO:0003419: growth plate cartilage chondrocyte proliferation

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

Notes

See also

References

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