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PMID:11687661

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Citation

Spencer, AG, Orita, S, Malone, CJ and Han, M (2001) A RHO GTPase-mediated pathway is required during P cell migration in Caenorhabditis elegans. Proc. Natl. Acad. Sci. U.S.A. 98:13132-7

Abstract

The Rho family of guanine triphosphate hydrolases controls various cellular processes, including cell migration. We describe here the demonstration of a role for a RhoA GTPase homologue during cell migration in Caenorhabditis elegans. We show that eliminating or reducing rho-1 gene function by using a dominant-negative transgene or dsRNA interference results in a severe defect in migration of hypodermal P cells to a ventral position. Biochemical and genetic data also suggest that unc-73, which encodes a Trio-like guanine nucleotide exchange factor, may act as an activator of rho-1 in the migration process. Mutations in let-502 ROCK, a homologue of a RhoA effector in mammals, also cause defects in P cell migration, suggesting that it may be one of several effectors acting downstream of rho-1 during P cell migration. Finally, we provide evidence to support the idea that other small Rac subfamily small GTPases act redundantly and in parallel to RHO-1 in this specific cell migration event.

Links

PubMed PMC60836 Online version:10.1073/pnas.241504098

Keywords

Animals; Caenorhabditis elegans/cytology; Caenorhabditis elegans/enzymology; Cell Movement; Guanine Nucleotide Exchange Factors/metabolism; Guanosine Diphosphate/metabolism; Guanosine Triphosphate/metabolism; Mice; Mice, Transgenic; Mutagenesis, Site-Directed; rho GTP-Binding Proteins/genetics; rho GTP-Binding Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CAEEL:C4ALD5

enables

GO:0005085: guanyl-nucleotide exchange factor activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

CAEEL:C4ALD5

involved_in

GO:0035022: positive regulation of Rac protein signal transduction

ECO:0000305: curator inference used in manual assertion

GO:0005085

P

Seeded From UniProt

complete

CAEEL:C4ALD5

involved_in

GO:0035025: positive regulation of Rho protein signal transduction

ECO:0000305: curator inference used in manual assertion

GO:0005085

P

Seeded From UniProt

complete

CAEEL:H2KYM2

enables

GO:0005085: guanyl-nucleotide exchange factor activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

CAEEL:H2KYM2

involved_in

GO:0035022: positive regulation of Rac protein signal transduction

ECO:0000305: curator inference used in manual assertion

GO:0005085

P

Seeded From UniProt

complete

CAEEL:H2KYM2

involved_in

GO:0035025: positive regulation of Rho protein signal transduction

ECO:0000305: curator inference used in manual assertion

GO:0005085

P

Seeded From UniProt

complete

CAEEL:H2KYM3

enables

GO:0005085: guanyl-nucleotide exchange factor activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

CAEEL:H2KYM3

involved_in

GO:0035022: positive regulation of Rac protein signal transduction

ECO:0000305: curator inference used in manual assertion

GO:0005085

P

Seeded From UniProt

complete

CAEEL:H2KYM3

involved_in

GO:0035025: positive regulation of Rho protein signal transduction

ECO:0000305: curator inference used in manual assertion

GO:0005085

P

Seeded From UniProt

complete

CAEEL:H2KYM4

enables

GO:0005085: guanyl-nucleotide exchange factor activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

CAEEL:H2KYM4

involved_in

GO:0035022: positive regulation of Rac protein signal transduction

ECO:0000305: curator inference used in manual assertion

GO:0005085

P

Seeded From UniProt

complete

CAEEL:H2KYM4

involved_in

GO:0035025: positive regulation of Rho protein signal transduction

ECO:0000305: curator inference used in manual assertion

GO:0005085

P

Seeded From UniProt

complete

CAEEL:O61528

enables

GO:0005085: guanyl-nucleotide exchange factor activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

CAEEL:O61528

involved_in

GO:0035022: positive regulation of Rac protein signal transduction

ECO:0000305: curator inference used in manual assertion

GO:0005085

P

Seeded From UniProt

complete

CAEEL:O61528

involved_in

GO:0035025: positive regulation of Rho protein signal transduction

ECO:0000305: curator inference used in manual assertion

GO:0005085

P

Seeded From UniProt

complete

CAEEL:Q7JNG4

enables

GO:0005085: guanyl-nucleotide exchange factor activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

CAEEL:Q7JNG4

involved_in

GO:0035022: positive regulation of Rac protein signal transduction

ECO:0000305: curator inference used in manual assertion

GO:0005085

P

Seeded From UniProt

complete

CAEEL:Q7JNG4

involved_in

GO:0035025: positive regulation of Rho protein signal transduction

ECO:0000305: curator inference used in manual assertion

GO:0005085

P

Seeded From UniProt

complete

CAEEL:Q7JNG6

enables

GO:0005085: guanyl-nucleotide exchange factor activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

CAEEL:Q7JNG6

involved_in

GO:0035022: positive regulation of Rac protein signal transduction

ECO:0000305: curator inference used in manual assertion

GO:0005085

P

Seeded From UniProt

complete

CAEEL:Q7JNG6

involved_in

GO:0035025: positive regulation of Rho protein signal transduction

ECO:0000305: curator inference used in manual assertion

GO:0005085

P

Seeded From UniProt

complete

CAEEL:Q7KPP4

enables

GO:0005085: guanyl-nucleotide exchange factor activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

CAEEL:Q7KPP4

involved_in

GO:0035022: positive regulation of Rac protein signal transduction

ECO:0000305: curator inference used in manual assertion

GO:0005085

P

Seeded From UniProt

complete

CAEEL:Q7KPP4

involved_in

GO:0035025: positive regulation of Rho protein signal transduction

ECO:0000305: curator inference used in manual assertion

GO:0005085

P

Seeded From UniProt

complete

CAEEL:Q95Q51

enables

GO:0005085: guanyl-nucleotide exchange factor activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

CAEEL:Q95Q51

involved_in

GO:0035022: positive regulation of Rac protein signal transduction

ECO:0000305: curator inference used in manual assertion

GO:0005085

P

Seeded From UniProt

complete

CAEEL:Q95Q51

involved_in

GO:0035025: positive regulation of Rho protein signal transduction

ECO:0000305: curator inference used in manual assertion

GO:0005085

P

Seeded From UniProt

complete


See also

References

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