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PMID:11684651

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Citation

Dottori, M, Gross, MK, Labosky, P and Goulding, M (2001) The winged-helix transcription factor Foxd3 suppresses interneuron differentiation and promotes neural crest cell fate. Development 128:4127-38

Abstract

The neural crest is a migratory cell population that gives rise to multiple cell types in the vertebrate embryo. The intrinsic determinants that segregate neural crest cells from multipotential dorsal progenitors within the neural tube are poorly defined. In this study, we show that the winged helix transcription factor Foxd3 is expressed in both premigratory and migratory neural crest cells. Foxd3 is genetically downstream of Pax3 and is not expressed in regions of Pax3 mutant mice that lack neural crest, implying that Foxd3 may regulate aspects of the neural crest differentiation program. We show that misexpression of Foxd3 in the chick neural tube promotes a neural crest-like phenotype and suppresses interneuron differentiation. Cells that ectopically express Foxd3 upregulate HNK1 and Cad7, delaminate and emigrate from the neural tube at multiple dorsoventral levels. Foxd3 does not induce Slug and RhoB, nor is its ability to promote a neural crest-like phenotype enhanced by co-expression of Slug. Together these results suggest Foxd3 can function independently of Slug and RhoB to promote the development of neural crest cells from neural tube progenitors.

Links

PubMed

Keywords

Animals; Antigens, CD57/genetics; Avian Proteins; Biological Markers; Cadherins/genetics; Cell Differentiation/genetics; Cell Movement; Chick Embryo; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Embryonic Induction/genetics; Forkhead Transcription Factors; Gene Expression Regulation, Developmental; Helix-Turn-Helix Motifs; Mice; Mice, Mutant Strains; Neural Crest/cytology; Neurons/cytology; Paired Box Transcription Factors; Repressor Proteins/genetics; Repressor Proteins/metabolism; Spinal Cord/embryology; Trans-Activators/genetics; Trans-Activators/metabolism; Transcription Factors/genetics; Transcription Factors/metabolism; rhoB GTP-Binding Protein/genetics

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:FOXD3

acts_upstream_of_or_within

GO:0006351: transcription, DNA-templated

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

MOUSE:FOXD3

part_of

GO:0005667: transcription regulator complex

ECO:0000304: author statement supported by traceable reference used in manual assertion

C

Seeded From UniProt

complete

CHICK:FOXD3

involved_in

GO:0043009: chordate embryonic development

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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