PMID:11672531

Citation	Porse, BT, Pedersen TA, , Xu, X, Lindberg, B, Wewer, UM, Friis-Hansen, L and Nerlov, C (2001) E2F repression by C/EBPalpha is required for adipogenesis and granulopoiesis in vivo. Cell 107:247-58
Abstract	The C/EBPalpha transcription factor is required for differentiation of adipocytes and neutrophil granulocytes, and controls cellular proliferation in vivo. To address the molecular mechanisms of C/EBPalpha action, we have identified C/EBPalpha mutants defective in repression of E2F-dependent transcription and found them to be impaired in their ability to suppress cellular proliferation, and to induce adipocyte differentiation in vitro. Using targeted mutagenesis of the mouse germline, we show that E2F repression-deficient C/EBPalpha alleles failed to support adipocyte and granulocyte differentiation in vivo. These results indicate that E2F repression by C/EBPalpha is critical for its ability to induce terminal differentiation, and thus provide genetic evidence that direct cell cycle control by a mammalian lineage-instructive transcription factor couples cellular growth arrest and differentiation.
Links	PubMed
Keywords	3T3 Cells; Adipocytes/cytology; Alleles; Amino Acid Sequence; Animals; Blotting, Northern; Blotting, Western; CCAAT-Enhancer-Binding Protein-alpha/chemistry; CCAAT-Enhancer-Binding Protein-alpha/metabolism; Cell Cycle Proteins; Cell Differentiation; Cell Division; DNA-Binding Proteins; E2F Transcription Factors; Female; Flow Cytometry; Genes, Reporter; Glutathione Transferase/metabolism; Granulocytes/cytology; Humans; Mice; Mice, Knockout; Mice, Transgenic; Molecular Sequence Data; Mutagenesis, Site-Directed; Ovary/metabolism; Protein Binding; Rats; Sequence Homology, Amino Acid; Tissue Distribution; Transcription Factors/chemistry; Transcription, Genetic
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
RAT:CEBPA	involved_in	GO:0045444: fat cell differentiation	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
RAT:CEBPA	involved_in	GO:0045892: negative regulation of transcription, DNA-templated	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
RAT:CEBPA	involved_in	GO:0008285: negative regulation of cell population proliferation	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
MOUSE:CEBPA	located_in	GO:0005634: nucleus	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
MOUSE:E2F1	involved_in	GO:0045599: negative regulation of fat cell differentiation	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
MOUSE:E2F1	involved_in	GO:0006351: transcription, DNA-templated	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
MOUSE:E2F1	enables	GO:0043565: sequence-specific DNA binding	ECO:0000314: direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
MOUSE:CEBPA	involved_in	GO:0045892: negative regulation of transcription, DNA-templated	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
MOUSE:CEBPA	involved_in	GO:0045444: fat cell differentiation	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
MOUSE:CEBPA	involved_in	GO:0008285: negative regulation of cell population proliferation	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
MOUSE:CEBPA	involved_in	GO:0030851: granulocyte differentiation	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
MOUSE:CEBPA	part_of	GO:0005634: nucleus	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
MOUSE:CEBPA	enables	GO:0043565: sequence-specific DNA binding	ECO:0000314: direct assay evidence used in manual assertion		F		Seeded From UniProt	complete

References

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