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PMID:11577099

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Citation

van der Vliet, HN, Sammels, MG, Leegwater, AC, Levels, JH, Reitsma, PH, Boers, W and Chamuleau, RA (2001) Apolipoprotein A-V: a novel apolipoprotein associated with an early phase of liver regeneration. J. Biol. Chem. 276:44512-20

Abstract

Liver regeneration in response to various forms of liver injury is a complex process, which ultimately results in restoration of the original liver mass and function. Because the underlying mechanisms that initiate this response are still incompletely defined, this study was aimed to identify novel factors. Liver genes that were up-regulated 6 h after 70% hepatectomy (PHx) in the rat were selected by cDNA subtractive hybridization. Besides known genes associated with cell proliferation, several novel genes were isolated. The novel gene that was most up-regulated was further studied. Its mRNA showed a liver-specific expression and encoded a protein comprising 367 amino acids. The mouse and human cDNA analogues were also isolated and appeared to be highly homologous. The human gene analogue was located at an apolipoprotein gene cluster on chromosome 11q23. The protein encoded by this gene had appreciable homology with apolipoproteins A-I and A-IV. Maximal expression of the gene in the rat liver and its gene product in rat plasma was observed 6 h after PHx. The protein was present in plasma fractions containing high density lipoprotein particles. Therefore, we have identified a novel apolipoprotein, designated apolipoprotein A-V, that is associated with an early phase of liver regeneration.

Links

PubMed Online version:10.1074/jbc.M106888200

Keywords

Amino Acid Sequence; Amino Acids/chemistry; Animals; Apolipoproteins; Apolipoproteins A/biosynthesis; Apolipoproteins A/blood; Apolipoproteins A/chemistry; Apolipoproteins A/genetics; Apolipoproteins A/metabolism; Base Sequence; Blotting, Northern; Blotting, Western; Chromatography, Gel; Chromosomes, Human, Pair 11; DNA, Complementary/metabolism; Humans; Liver/physiology; Male; Mice; Models, Genetic; Molecular Sequence Data; Nucleic Acid Hybridization; RNA, Messenger/metabolism; Rats; Rats, Wistar; Regeneration; Sequence Homology, Amino Acid; Time Factors; Tissue Distribution; Up-Regulation

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:APOA5

located_in

GO:0005576: extracellular region

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:APOA5

involved_in

GO:0031100: animal organ regeneration

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:APOA5

involved_in

GO:0042246: tissue regeneration

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:APOA5

part_of

GO:0005576: extracellular region

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

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