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PMID:11555281

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Citation

Sheikh, J, Hicks, S, Dall'Agnol, M, Phillips, AD and Nataro, JP (2001) Roles for Fis and YafK in biofilm formation by enteroaggregative Escherichia coli. Mol. Microbiol. 41:983-97

Abstract

Enteroaggregative Escherichia coli (EAEC) forms thick biofilms on the intestinal mucosa. Here, we show that most EAEC strains form a biofilm on glass or plastic surfaces when grown in cell culture medium with high sugar and osmolarity. Biofilm-forming ability in two prototype EAEC strains required aggregative adherence fimbriae (AAF), although many other EAEC strains that do not express AAF also developed biofilms under these conditions. Ten thousand transposon mutants of EAEC strain 042 were isolated, and 100 were found to be deficient in biofilm formation. Of these, 93 were either deficient in in vitro growth or mapped to genes known to be required for AAF/II expression. Of the seven remaining insertions, five mapped to one of two unsuspected loci. Two insertions involved the E. coli chromosomal fis gene, a DNA-binding protein that is involved in growth phase-dependent regulation. Using reverse transcription-polymerase chain reaction (RT-PCR), we determined that the effect of fis was at the level of transcription of the AAF/II activator aggR. Biofilm formation also required the product of the yafK gene, which is predicted to encode a secreted 28 kDa protein. The yafK product is required for transcription of AAF/II-encoding genes. Our data do not suggest a role for type 1 fimbriae or motility in biofilm formation. EAEC appears to form a novel biofilm, which may be mediated solely by AAF and may reflect its interactions with the intestinal mucosa.

Links

PubMed

Keywords

Adhesins, Escherichia coli/genetics; Adhesins, Escherichia coli/metabolism; Bacterial Adhesion; Biofilms/growth & development; Biopsy; Carrier Proteins/genetics; Carrier Proteins/metabolism; Child; Child, Preschool; Colon/microbiology; Culture Media; Escherichia coli/genetics; Escherichia coli/physiology; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Factor For Inversion Stimulation Protein; Glass; Humans; Integration Host Factors; Intestinal Mucosa/microbiology; Microscopy, Electron, Scanning; Plastics

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

PSEAE:Q9HXN9

enables

GO:0008234: cysteine-type peptidase activity

ECO:0000250: sequence similarity evidence used in manual assertion

RefSeq:NP_414759.1

F

Seeded From UniProt

complete

PSEAE:Q9HXN9

enables

GO:0071972: peptidoglycan L,D-transpeptidase activity

ECO:0000250: sequence similarity evidence used in manual assertion

RefSeq:NP_414759.1

F

Seeded From UniProt

complete

ECO44:D3H539

GO:0090609: single-species submerged biofilm formation

ECO:0000315:

P

Figure 4. shows that strain with TnphoA inserted into aafA gene was biofilm negative.

complete
CACAO 6029

ECO44:D3H539

involved_in

GO:0090609: single-species submerged biofilm formation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ECO44:D3GYQ2

GO:0090609: single-species submerged biofilm formation

ECO:0000315:

P

Figure 4. shows that fis mutant was biofilm negative.

complete
CACAO 6030

ECO44:D3GYQ2

involved_in

GO:0090609: single-species submerged biofilm formation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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