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PMID:11478948

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Citation

Gachon, F, Gaudray, G, Thébault, S, Basbous, J, Koffi, JA, Devaux, C and Mesnard, J (2001) The cAMP response element binding protein-2 (CREB-2) can interact with the C/EBP-homologous protein (CHOP). FEBS Lett. 502:57-62

Abstract

cAMP response element binding protein-2 (CREB-2) is a basic leucine zipper (bZIP) factor that was originally described as a repressor of CRE-dependent transcription but that can also act as a transcriptional activator. Moreover, CREB-2 is able to function in association with the viral Tax protein as an activator of the human T-cell leukemia virus type I (HTLV-I) promoter. Here we show that CREB-2 is able to interact with C/EBP-homologous protein (CHOP), a bZIP transcription factor known to inhibit CAAT/enhancer-dependent transcription. Cotransfection of CHOP with CREB-2 results in decreased activation driven by the cellular CRE motif or the HTLV-I proximal Tax-responsive element, confirming that CREB-2 and CHOP can interact with each other in vivo.

Links

PubMed

Keywords

Activating Transcription Factor 4; Animals; CCAAT-Enhancer-Binding Proteins/genetics; CCAAT-Enhancer-Binding Proteins/metabolism; COS Cells; Cell Line; Cell Nucleus/metabolism; Cercopithecus aethiops; Gene Expression Regulation, Viral; Gene Products, tat/metabolism; HIV-1/genetics; Human T-lymphotropic virus 1/genetics; Humans; Leucine Zippers; Promoter Regions, Genetic; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism; Saccharomyces cerevisiae; Transcription Factor CHOP; Transcription Factors/genetics; Transcription Factors/metabolism; Transcription, Genetic; Two-Hybrid System Techniques; tat Gene Products, Human Immunodeficiency Virus

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:ATF4

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:ATF4

enables

GO:0001085: RNA polymerase II transcription factor binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P35638

F

Seeded From UniProt

complete

HUMAN:ATF4

enables

GO:0043522: leucine zipper domain binding

ECO:0000314: direct assay evidence used in manual assertion

F

  • has_input:(UniProtKB:P35638)

Seeded From UniProt

complete

HUMAN:DDIT3

enables

GO:0008140: cAMP response element binding protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P18848

F

Seeded From UniProt

complete

HUMAN:DDIT3

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:DDIT3

involved_in

GO:0032792: negative regulation of CREB transcription factor activity

ECO:0000316: genetic interaction evidence used in manual assertion

UniProtKB:P18848

P

  • has_input:(UniProtKB:P18848)

Seeded From UniProt

complete

HUMAN:DDIT3

enables

GO:0043522: leucine zipper domain binding

ECO:0000314: direct assay evidence used in manual assertion

F

  • has_input:(UniProtKB:P18848)

Seeded From UniProt

complete

HUMAN:ATF4

enables

GO:0043522: leucine zipper domain binding

ECO:0000314: direct assay evidence used in manual assertion

F

has_direct_input:(UniProtKB:P35638)

Seeded From UniProt

complete

HUMAN:ATF4

enables

GO:0000981: DNA-binding transcription factor activity, RNA polymerase II-specific

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:ATF4

enables

GO:0001085: RNA polymerase II transcription factor binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P35638

F

part_of:(GO:0000122)

Seeded From UniProt

complete

HUMAN:ATF4

part_of

GO:1990617: CHOP-ATF4 complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:ATF4

involved_in

GO:0045944: positive regulation of transcription by RNA polymerase II

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:ATF4

part_of

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:DDIT3

enables

GO:0043522: leucine zipper domain binding

ECO:0000314: direct assay evidence used in manual assertion

F

has_direct_input:(UniProtKB:P18848)

Seeded From UniProt

complete

HUMAN:DDIT3

enables

GO:0008140: cAMP response element binding protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P18848

F

part_of:(GO:0000122)

Seeded From UniProt

complete

HUMAN:DDIT3

part_of

GO:1990617: CHOP-ATF4 complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:DDIT3

involved_in

GO:0000122: negative regulation of transcription by RNA polymerase II

ECO:0000316: genetic interaction evidence used in manual assertion

UniProtKB:P18848

P

Seeded From UniProt

complete

HUMAN:DDIT3

involved_in

GO:0032792: negative regulation of CREB transcription factor activity

ECO:0000316: genetic interaction evidence used in manual assertion

UniProtKB:P18848

P

has_regulation_target:(UniProtKB:P18848)

Seeded From UniProt

complete

HUMAN:DDIT3

part_of

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

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