PMID:11455386

Citation	Lieb, JD, Liu, X, Botstein, D and Brown, PO (2001) Promoter-specific binding of Rap1 revealed by genome-wide maps of protein-DNA association. Nat. Genet. 28:327-34
Abstract	We determined the distribution of repressor-activator protein 1 (Rap1) and the accessory silencing proteins Sir2, Sir3 and Sir4 in vivo on the entire yeast genome, at a resolution of 2 kb. Rap1 is central to the cellular economy during rapid growth, targeting 294 loci, about 5% of yeast genes, and participating in the activation of 37% of all RNA polymerase II initiation events in exponentially growing cells. Although the DNA sequence recognized by Rap1 is found in both coding and intergenic sequences, the binding of Rap1 to the genome was highly specific to intergenic regions with the potential to act as promoters. This global phenomenon, which may be a general characteristic of sequence-specific transcriptional factors, indicates the existence of a genome-wide molecular mechanism for marking promoter regions.
Links	PubMed Online version:10.1038/ng569
Keywords	Binding Sites/genetics; DNA/metabolism; DNA, Intergenic/metabolism; DNA-Binding Proteins/metabolism; Fungal Proteins/metabolism; Gene Expression Regulation/physiology; Genes, Fungal/physiology; Genome, Fungal; Glycolysis/genetics; Histone Deacetylases/metabolism; Open Reading Frames/physiology; Physical Chromosome Mapping/methods; Promoter Regions, Genetic/physiology; Protein Binding/physiology; RNA Polymerase II/metabolism; Ribosomal Proteins/genetics; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/metabolism; Silent Information Regulator Proteins, Saccharomyces cerevisiae; Sirtuin 2; Sirtuins; Telomere/metabolism; Trans-Activators/metabolism
edit table

Annotations

Gene product	Qualifier	GO ID	GO term name	Evidence Code	with/from	Aspect	Notes	Status
edit table

References

See Help:References for how to manage references in GONUTS.