PMID:11423561

Citation	Kobayashi, K, Uchida, S, Mizutani, S, Sasaki, S and Marumo, F (2001) Intrarenal and cellular localization of CLC-K2 protein in the mouse kidney. J. Am. Soc. Nephrol. 12:1327-34
Abstract	CLC-K2, a kidney-specific member of the CLC chloride channel family, is thought to play an important role in the transepithelial Cl(-) transport in the kidney. This consensus was first reached shortly after it was demonstrated that the mutations of the human CLCNKB gene resulted in Bartter's syndrome type III. To clarify the pathogenesis, the exact intrarenal and cellular localization of CLC-K2 by immunohistochemistry of the Clcnk1-/- mouse kidney were investigated by use of an anti-CLC-K antibody that recognized both CLC-K1 and CLC-K2. CLC-K2 is expressed in the thick ascending limb of Henle's loop and distal tubules, where it is localized to the basolateral membranes. The localization of CLC-K2 to these nephron segments strongly implies that CLC-K2 confers the basolateral chloride conductance in the thick ascending limb of Henle's loop and distal tubules, where Cl(-) is taken up by the bumetanide-sensitive Na-K-2Cl cotransporter or the thiazide-sensitive Na-Cl cotransporter at the apical membranes. CLC-K2 expression was also shown to extend into the connecting tubule in the basolateral membrane. CLC-K2 was found in basolateral membranes of the type A intercalated cells residing along the collecting duct. This localization strongly suggests that CLC-K2 confers the basolateral conductance in the type A intercalated cells where Cl(-) is taken up by the anion exchanger in exchange for HCO(3)(-) at the basolateral membranes. These aspects of CLC-K2 localization suggest that CLC-K2 is important in Cl(-) transport in the distal nephron segments.
Links	PubMed
Keywords	Animals; Anion Transport Proteins; Chloride Channels/genetics; Chloride Channels/metabolism; Enzyme-Linked Immunosorbent Assay; Immunoblotting; Immunohistochemistry; Kidney/cytology; Kidney/metabolism; Membrane Proteins; Mice; Mice, Knockout/genetics; Reference Values; Tissue Distribution
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
MOUSE:VATE1	located_in	GO:0016324: apical plasma membrane	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
MOUSE:S12A1	located_in	GO:0016324: apical plasma membrane	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
MOUSE:AQP2	located_in	GO:0016324: apical plasma membrane	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
MOUSE:S12A3	located_in	GO:0016324: apical plasma membrane	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
MOUSE:NAC1	located_in	GO:0016323: basolateral plasma membrane	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
MOUSE:CLCKA	located_in	GO:0016323: basolateral plasma membrane	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
MOUSE:CLCKA	involved_in	GO:0072053: renal inner medulla development	ECO:0000315: mutant phenotype evidence used in manual assertion	MGI:MGI:2148676	P		Seeded From UniProt	complete

References

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