GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:11387221

From GONUTS
Jump to: navigation, search
Citation

Nakai, A and Ishikawa, T (2001) Cell cycle transition under stress conditions controlled by vertebrate heat shock factors. EMBO J. 20:2885-95

Abstract

The roles of heat shock transcription factors (HSFs) under physiological conditions have recently become the focus of intense study. We generated avian cells lacking two heat-inducible HSFs, HSF1 and HSF3. In addition to complete loss of activation of heat shock genes under stress conditions, these cells exhibited a marked reduction in Hsp90alpha expression under normal growth conditions. Reduction in Hsp90alpha expression caused instability of a cyclin-dependent kinase, Cdc2, and cell cycle progression was blocked mainly at the G2 phase, but also at G1 phase even at mild heat shock temperatures. Restoration of Hsp90alpha expression rescued the temperature sensitivity without induction of HSPS: We demonstrated for the first time a molecular target affected by heat shock in vivo that causes cell cycle arrest in vertebrates and a novel mechanism of stress resistance controlled by vertebrate HSFS:

Links

PubMed PMC125499 Online version:10.1093/emboj/20.11.2885

Keywords

Animals; Avian Proteins; B-Lymphocytes/cytology; B-Lymphocytes/physiology; CDC2-CDC28 Kinases; Cell Cycle/physiology; Chickens; Cloning, Molecular; Cyclin-Dependent Kinase 2; Cyclin-Dependent Kinases/metabolism; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; HSP90 Heat-Shock Proteins/genetics; HSP90 Heat-Shock Proteins/metabolism; Heat-Shock Proteins/genetics; Heat-Shock Proteins/metabolism; Hot Temperature; Kinetics; Liver/metabolism; Protein-Serine-Threonine Kinases/metabolism; Recombinant Proteins/metabolism; Trans-Activators/genetics; Trans-Activators/metabolism; Transcription Factors/genetics; Transcription Factors/metabolism; Transfection; Vertebrates

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CHICK:F1NBD7

involved_in

GO:0000086: G2/M transition of mitotic cell cycle

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:F1NBD7

involved_in

GO:0034605: cellular response to heat

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:HSF1

involved_in

GO:0008284: positive regulation of cell population proliferation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:HSF1

involved_in

GO:0010628: positive regulation of gene expression

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:HSF1

enables

GO:0003677: DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

CHICK:HSF1

involved_in

GO:1902808: positive regulation of cell cycle G1/S phase transition

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:HSF1

involved_in

GO:1902751: positive regulation of cell cycle G2/M phase transition

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:HSF3

involved_in

GO:0008284: positive regulation of cell population proliferation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:HSF3

involved_in

GO:0010628: positive regulation of gene expression

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:HSF3

enables

GO:0003677: DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

CHICK:HSF3

involved_in

GO:1902808: positive regulation of cell cycle G1/S phase transition

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:HSF3

involved_in

GO:1902751: positive regulation of cell cycle G2/M phase transition

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:CDK1

involved_in

GO:0000086: G2/M transition of mitotic cell cycle

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:CDK1

involved_in

GO:0034605: cellular response to heat

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:HS90A

involved_in

GO:0050821: protein stabilization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:HS90A

involved_in

GO:1902751: positive regulation of cell cycle G2/M phase transition

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:HS90A

involved_in

GO:0008284: positive regulation of cell population proliferation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:HSF1

involved_in

GO:1902808: positive regulation of cell cycle G1/S phase transition

ECO:0000315: mutant phenotype evidence used in manual assertion

P

part_of:(GO:0034605)

Seeded From UniProt

complete

CHICK:HSF1

involved_in

GO:1902751: positive regulation of cell cycle G2/M phase transition

ECO:0000315: mutant phenotype evidence used in manual assertion

P

part_of:(GO:0034605)

Seeded From UniProt

complete

CHICK:HSF1

involved_in

GO:0010628: positive regulation of gene expression

ECO:0000315: mutant phenotype evidence used in manual assertion

P

part_of:(GO:0034605)|part_of:(GO:1903936)|part_of:(GO:0071417)|has_regulation_target(UniProtKB:P11501)

Seeded From UniProt

complete

CHICK:HSF1

involved_in

GO:0008284: positive regulation of cell population proliferation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

part_of:(GO:0034605)|part_of:(GO:0071478)|part_of:(GO:1903936)

Seeded From UniProt

complete

CHICK:HSF1

involved_in

GO:0034605: cellular response to heat

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:HSF1

enables

GO:0003677: DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

part_of:(GO:0034605)

Seeded From UniProt

complete

CHICK:HSF3

involved_in

GO:1902751: positive regulation of cell cycle G2/M phase transition

ECO:0000315: mutant phenotype evidence used in manual assertion

P

part_of:(GO:0034605)

Seeded From UniProt

complete

CHICK:HSF3

involved_in

GO:1902808: positive regulation of cell cycle G1/S phase transition

ECO:0000315: mutant phenotype evidence used in manual assertion

P

part_of:(GO:0034605)

Seeded From UniProt

complete

CHICK:HSF3

involved_in

GO:0008284: positive regulation of cell population proliferation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

part_of:(GO:0034605)|part_of:(GO:0071478)|part_of:(GO:1903936)

Seeded From UniProt

complete

CHICK:HSF3

involved_in

GO:0010628: positive regulation of gene expression

ECO:0000315: mutant phenotype evidence used in manual assertion

P

part_of:(GO:0034605)|part_of:(GO:1903936)|part_of:(GO:0071417)|has_regulation_target(UniProtKB:P11501)

Seeded From UniProt

complete

CHICK:HSF3

enables

GO:0003677: DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

part_of:(GO:0034605)

Seeded From UniProt

complete

CHICK:HSF3

involved_in

GO:0034605: cellular response to heat

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

Notes

See also

References

See Help:References for how to manage references in GONUTS.