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PMID:11350937

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Citation

Gutknecht, R, Beutler, R, Garcia-Alles, LF, Baumann, U and Erni, B (2001) The dihydroxyacetone kinase of Escherichia coli utilizes a phosphoprotein instead of ATP as phosphoryl donor. EMBO J. 20:2480-6

Abstract

The dihydroxyacetone kinase (DhaK) of Escherichia coli consists of three soluble protein subunits. DhaK (YcgT; 39.5 kDa) and DhaL (YcgS; 22.6 kDa) are similar to the N- and C-terminal halves of the ATP-dependent DhaK ubiquitous in bacteria, animals and plants. The homodimeric DhaM (YcgC; 51.6 kDa) consists of three domains. The N-terminal dimerization domain has the same fold as the IIA domain (PDB code 1PDO) of the mannose transporter of the bacterial phosphoenolpyruvate:sugar phosphotransferase system (PTS). The middle domain is similar to HPr and the C-terminus is similar to the N-terminal domain of enzyme I (EI) of the PTS. DhaM is phosphorylated three times by phosphoenolpyruvate in an EI- and HPr-dependent reaction. DhaK and DhaL are not phosphorylated. The IIA domain of DhaM, instead of ATP, is the phosphoryl donor to dihydroxyacetone (Dha). Unlike the carbohydrate-specific transporters of the PTS, DhaK, DhaL and DhaM have no transport activity.

Links

PubMed PMC125457 Online version:10.1093/emboj/20.10.2480

Keywords

Adenosine Triphosphate/metabolism; Amino Acid Sequence; Binding Sites; Escherichia coli/enzymology; Genes, Bacterial; Molecular Sequence Data; Mutagenesis; Operon; Phosphates/metabolism; Phosphoproteins/metabolism; Phosphorylation; Phosphotransferases/metabolism; Phosphotransferases (Alcohol Group Acceptor)/biosynthesis; Phosphotransferases (Alcohol Group Acceptor)/chemistry; Phosphotransferases (Alcohol Group Acceptor)/genetics; Phosphotransferases (Alcohol Group Acceptor)/metabolism; Protein Structure, Secondary; Sequence Homology, Amino Acid

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ECOLI:DHAL

contributes_to

GO:0016740: transferase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ECOLI:PTHP

GO:0042327 : positive regulation of phosphorylation

ECO:0000314:

P

See Figure 4B.

complete

ECOLI:DHAL

Contributes to

GO:0016740: transferase activity

ECO:0000314:

F

See Fig. 4C.

complete

ECOLI:DHAM

Contributes to

GO:0016740: transferase activity

ECO:0000314:

F

See Fig. 4C.

complete

ECOLI:DHAK

Contributes to

GO:0016740: transferase activity

ECO:0000314:

F

See Fig. 4C.

complete


See also

References

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