PMID:11333230

Citation	Du, H and Chalfie, M (2001) Genes regulating touch cell development in Caenorhabditis elegans. Genetics 158:197-207
Abstract	To identify genes regulating the development of the six touch receptor neurons, we screened the F(2) progeny of mutated animals expressing an integrated mec-2::gfp transgene that is expressed mainly in these touch cells. From 2638 mutated haploid genomes, we obtained 11 mutations representing 11 genes that affected the production, migration, or outgrowth of the touch cells. Eight of these mutations were in known genes, and 2 defined new genes (mig-21 and vab-15). The mig-21 mutation is the first known to affect the asymmetry of the migrations of Q neuroblasts, the cells that give rise to two of the six touch cells. vab-15 is a msh-like homeobox gene that appears to be needed for the proper production of touch cell precursors, since vab-15 animals lacked the four more posterior touch cells. The remaining touch cells (the ALM cells) were present but mispositioned. A similar touch cell phenotype is produced by mutations in lin-32. A more severe phenotype; i.e., animals often lacked ALM cells, was seen in lin-32 vab-15 double mutants, suggesting that these genes acted redundantly in ALM differentiation. In addition to the touch cell abnormalities, vab-15 animals variably exhibit embryonic or larval lethality, cell degenerations, malformation of the posterior body, uncoordinated movement, and defective egg laying.
Links	PubMed PMC1461620
Keywords	Alleles; Amino Acid Sequence; Animals; Base Sequence; Caenorhabditis elegans/cytology; Caenorhabditis elegans/enzymology; Caenorhabditis elegans/genetics; Cell Differentiation/genetics; Cell Lineage; DNA Primers; Embryo, Nonmammalian/cytology; Genes, Homeobox; Humans; Molecular Sequence Data; Mutation; Reverse Transcriptase Polymerase Chain Reaction; Sequence Homology, Amino Acid
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
CAEEL:VAB15	located_in	GO:0005634: nucleus	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
CAEEL:VAB15	involved_in	GO:0007626: locomotory behavior	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
CAEEL:VAB15	involved_in	GO:0007567: parturition	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
CAEEL:VAB15	enables	GO:0003700: DNA-binding transcription factor activity	ECO:0000304: author statement supported by traceable reference used in manual assertion		F		Seeded From UniProt	complete
CAEEL:VAB15	involved_in	GO:0048598: embryonic morphogenesis	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
CAEEL:VAB15	enables	GO:0043565: sequence-specific DNA binding	ECO:0000304: author statement supported by traceable reference used in manual assertion		F		Seeded From UniProt	complete

References

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