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PMID:11319236

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Citation

Ollagnier-de-Choudens, S, Mattioli, T, Takahashi, Y and Fontecave, M (2001) Iron-sulfur cluster assembly: characterization of IscA and evidence for a specific and functional complex with ferredoxin. J. Biol. Chem. 276:22604-7

Abstract

The synthesis of iron-sulfur clusters in Escherichia coli is believed to require a complex protein machinery encoded by the isc (iron-sulfur cluster) operon. The product of one member of this operon, IscA, has been overexpressed, purified, and characterized. It can assemble an air-sensitive [2Fe-2S] cluster as shown by UV-visible and resonance Raman spectroscopy. The metal form but not the apoform of IscA binds ferredoxin, another member of the isc operon, selectively, allowing transfer of iron and sulfide from IscA to ferredoxin and formation of the [2Fe-2S] holoferredoxin. These results thus suggest that IscA is involved in ferredoxin cluster assembly and activation. This is an important function because a functional ferredoxin is required for maturation of other cellular Fe-S proteins.

Links

PubMed Online version:10.1074/jbc.M102902200

Keywords

Bacterial Proteins/isolation & purification; Bacterial Proteins/metabolism; Base Sequence; DNA Primers; Electrophoresis, Polyacrylamide Gel; Ferredoxins/metabolism; Iron-Sulfur Proteins/metabolism; Operon; Protein Binding; Recombinant Proteins/isolation & purification; Recombinant Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ECOLI:FER

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P0AAC8

F

Seeded From UniProt

complete

ECOLI:ISCA

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P0A9R4

F

Seeded From UniProt

complete

ECOLI:ISCA

enables

GO:0051537: 2 iron, 2 sulfur cluster binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ECOLI:ISCA

involved_in

GO:0016226: iron-sulfur cluster assembly

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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