PMID:11311163

Citation	Lowe, LA, Yamada, S and Kuehn, MR (2001) Genetic dissection of nodal function in patterning the mouse embryo. Development 128:1831-43
Abstract	Loss-of-function analysis has shown that the transforming growth factor-like signaling molecule nodal is essential for mouse mesoderm development. However, definitive proof of nodal function in other developmental processes in the mouse embryo has been lacking because the null mutation blocks gastrulation. We describe the generation and analysis of a hypomorphic nodal allele. Mouse embryos heterozygous for the hypomorphic allele and a null allele undergo gastrulation but then display abnormalities that fall into three distinct mutant phenotypic classes, which may result from expression levels falling below critical thresholds in one or more domains of nodal expression. Our analysis of each of these classes provides conclusive evidence for nodal-mediated regulation of several developmental processes in the mouse embryo, beyond its role in mesoderm formation. We find that nodal signaling is required for correct positioning of the anteroposterior axis, normal anterior and midline patterning, and the left-right asymmetric development of the heart, vasculature, lungs and stomach.
Links	PubMed
Keywords	Alleles; Animals; Body Patterning/genetics; Body Patterning/physiology; Female; Gastrula/physiology; Gene Expression Regulation, Developmental; Heterozygote; Male; Mice; Mice, Inbred C57BL; Mice, Knockout; Mutation; Nodal Protein; Phenotype; Signal Transduction; Transforming Growth Factor beta/genetics; Transforming Growth Factor beta/physiology
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
MOUSE:NODAL	involved_in	GO:0048382: mesendoderm development	ECO:0000315: mutant phenotype evidence used in manual assertion	MGI:MGI:2158732	P		Seeded From UniProt	complete
MOUSE:NODAL	involved_in	GO:0048546: digestive tract morphogenesis	ECO:0000315: mutant phenotype evidence used in manual assertion	MGI:MGI:2158732	P		Seeded From UniProt	complete
MOUSE:NODAL	involved_in	GO:0048701: embryonic cranial skeleton morphogenesis	ECO:0000315: mutant phenotype evidence used in manual assertion	MGI:MGI:2158732	P		Seeded From UniProt	complete
MOUSE:NODAL	involved_in	GO:0048729: tissue morphogenesis	ECO:0000315: mutant phenotype evidence used in manual assertion	MGI:MGI:2158732	P		Seeded From UniProt	complete
MOUSE:NODAL	involved_in	GO:0048859: formation of anatomical boundary	ECO:0000315: mutant phenotype evidence used in manual assertion	MGI:MGI:2158732	P		Seeded From UniProt	complete
MOUSE:NODAL	involved_in	GO:0055123: digestive system development	ECO:0000315: mutant phenotype evidence used in manual assertion	MGI:MGI:2158732	P		Seeded From UniProt	complete
MOUSE:NODAL	involved_in	GO:0060460: left lung morphogenesis	ECO:0000315: mutant phenotype evidence used in manual assertion	MGI:MGI:2158732	P		Seeded From UniProt	complete
MOUSE:NODAL	involved_in	GO:0007399: nervous system development	ECO:0000315: mutant phenotype evidence used in manual assertion	MGI:MGI:2158732	P		Seeded From UniProt	complete
MOUSE:NODAL	involved_in	GO:0007420: brain development	ECO:0000315: mutant phenotype evidence used in manual assertion	MGI:MGI:2158732	P		Seeded From UniProt	complete
MOUSE:NODAL	involved_in	GO:0007492: endoderm development	ECO:0000315: mutant phenotype evidence used in manual assertion	MGI:MGI:2158732	P		Seeded From UniProt	complete
MOUSE:NODAL	involved_in	GO:0007507: heart development	ECO:0000315: mutant phenotype evidence used in manual assertion	MGI:MGI:2158732	P		Seeded From UniProt	complete
MOUSE:NODAL	involved_in	GO:0009880: embryonic pattern specification	ECO:0000315: mutant phenotype evidence used in manual assertion	MGI:MGI:2158732	P		Seeded From UniProt	complete
MOUSE:NODAL	involved_in	GO:0009948: anterior/posterior axis specification	ECO:0000315: mutant phenotype evidence used in manual assertion	MGI:MGI:2158732	P		Seeded From UniProt	complete
MOUSE:NODAL	involved_in	GO:0009952: anterior/posterior pattern specification	ECO:0000315: mutant phenotype evidence used in manual assertion	MGI:MGI:2158732	P		Seeded From UniProt	complete
MOUSE:NODAL	involved_in	GO:0030324: lung development	ECO:0000315: mutant phenotype evidence used in manual assertion	MGI:MGI:2158732	P		Seeded From UniProt	complete
MOUSE:NODAL	involved_in	GO:0033505: floor plate morphogenesis	ECO:0000315: mutant phenotype evidence used in manual assertion	MGI:MGI:2158732	P		Seeded From UniProt	complete
MOUSE:NODAL	involved_in	GO:0035050: embryonic heart tube development	ECO:0000315: mutant phenotype evidence used in manual assertion	MGI:MGI:2158732	P		Seeded From UniProt	complete
MOUSE:NODAL	involved_in	GO:0007368: determination of left/right symmetry	ECO:0000315: mutant phenotype evidence used in manual assertion	MGI:MGI:2158732	P		Seeded From UniProt	complete
MOUSE:NODAL	involved_in	GO:0007369: gastrulation	ECO:0000315: mutant phenotype evidence used in manual assertion	MGI:MGI:2158732	P		Seeded From UniProt	complete
MOUSE:NODAL	involved_in	GO:0001701: in utero embryonic development	ECO:0000315: mutant phenotype evidence used in manual assertion	MGI:MGI:2158732	P		Seeded From UniProt	complete
MOUSE:NODAL	involved_in	GO:0001707: mesoderm formation	ECO:0000315: mutant phenotype evidence used in manual assertion	MGI:MGI:2158732	P		Seeded From UniProt	complete
MOUSE:NODAL	involved_in	GO:0001842: neural fold formation	ECO:0000315: mutant phenotype evidence used in manual assertion	MGI:MGI:2158732	P		Seeded From UniProt	complete
MOUSE:NODAL	involved_in	GO:0001889: liver development	ECO:0000315: mutant phenotype evidence used in manual assertion	MGI:MGI:2158732	P		Seeded From UniProt	complete
MOUSE:NODAL	involved_in	GO:0001944: vasculature development	ECO:0000315: mutant phenotype evidence used in manual assertion	MGI:MGI:2158732	P		Seeded From UniProt	complete

References

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