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PMID:11311163

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Citation

Lowe, LA, Yamada, S and Kuehn, MR (2001) Genetic dissection of nodal function in patterning the mouse embryo. Development 128:1831-43

Abstract

Loss-of-function analysis has shown that the transforming growth factor-like signaling molecule nodal is essential for mouse mesoderm development. However, definitive proof of nodal function in other developmental processes in the mouse embryo has been lacking because the null mutation blocks gastrulation. We describe the generation and analysis of a hypomorphic nodal allele. Mouse embryos heterozygous for the hypomorphic allele and a null allele undergo gastrulation but then display abnormalities that fall into three distinct mutant phenotypic classes, which may result from expression levels falling below critical thresholds in one or more domains of nodal expression. Our analysis of each of these classes provides conclusive evidence for nodal-mediated regulation of several developmental processes in the mouse embryo, beyond its role in mesoderm formation. We find that nodal signaling is required for correct positioning of the anteroposterior axis, normal anterior and midline patterning, and the left-right asymmetric development of the heart, vasculature, lungs and stomach.

Links

PubMed

Keywords

Alleles; Animals; Body Patterning/genetics; Body Patterning/physiology; Female; Gastrula/physiology; Gene Expression Regulation, Developmental; Heterozygote; Male; Mice; Mice, Inbred C57BL; Mice, Knockout; Mutation; Nodal Protein; Phenotype; Signal Transduction; Transforming Growth Factor beta/genetics; Transforming Growth Factor beta/physiology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:NODAL

involved_in

GO:0048382: mesendoderm development

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:2158732

P

Seeded From UniProt

complete

MOUSE:NODAL

involved_in

GO:0048546: digestive tract morphogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:2158732

P

Seeded From UniProt

complete

MOUSE:NODAL

involved_in

GO:0048701: embryonic cranial skeleton morphogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:2158732

P

Seeded From UniProt

complete

MOUSE:NODAL

involved_in

GO:0048729: tissue morphogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:2158732

P

Seeded From UniProt

complete

MOUSE:NODAL

involved_in

GO:0048859: formation of anatomical boundary

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:2158732

P

Seeded From UniProt

complete

MOUSE:NODAL

involved_in

GO:0055123: digestive system development

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:2158732

P

Seeded From UniProt

complete

MOUSE:NODAL

involved_in

GO:0060460: left lung morphogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:2158732

P

Seeded From UniProt

complete

MOUSE:NODAL

involved_in

GO:0007399: nervous system development

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:2158732

P

Seeded From UniProt

complete

MOUSE:NODAL

involved_in

GO:0007420: brain development

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:2158732

P

Seeded From UniProt

complete

MOUSE:NODAL

involved_in

GO:0007492: endoderm development

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:2158732

P

Seeded From UniProt

complete

MOUSE:NODAL

involved_in

GO:0007507: heart development

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:2158732

P

Seeded From UniProt

complete

MOUSE:NODAL

involved_in

GO:0009880: embryonic pattern specification

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:2158732

P

Seeded From UniProt

complete

MOUSE:NODAL

involved_in

GO:0009948: anterior/posterior axis specification

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:2158732

P

Seeded From UniProt

complete

MOUSE:NODAL

involved_in

GO:0009952: anterior/posterior pattern specification

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:2158732

P

Seeded From UniProt

complete

MOUSE:NODAL

involved_in

GO:0030324: lung development

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:2158732

P

Seeded From UniProt

complete

MOUSE:NODAL

involved_in

GO:0033505: floor plate morphogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:2158732

P

Seeded From UniProt

complete

MOUSE:NODAL

involved_in

GO:0035050: embryonic heart tube development

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:2158732

P

Seeded From UniProt

complete

MOUSE:NODAL

involved_in

GO:0007368: determination of left/right symmetry

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:2158732

P

Seeded From UniProt

complete

MOUSE:NODAL

involved_in

GO:0007369: gastrulation

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:2158732

P

Seeded From UniProt

complete

MOUSE:NODAL

involved_in

GO:0001701: in utero embryonic development

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:2158732

P

Seeded From UniProt

complete

MOUSE:NODAL

involved_in

GO:0001707: mesoderm formation

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:2158732

P

Seeded From UniProt

complete

MOUSE:NODAL

involved_in

GO:0001842: neural fold formation

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:2158732

P

Seeded From UniProt

complete

MOUSE:NODAL

involved_in

GO:0001889: liver development

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:2158732

P

Seeded From UniProt

complete

MOUSE:NODAL

involved_in

GO:0001944: vasculature development

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:2158732

P

Seeded From UniProt

complete


See also

References

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