PMID:11290718

Citation	Dobie, KW, Kennedy, CD, Velasco, VM, McGrath, TL, Weko, J, Patterson, RW and Karpen, GH (2001) Identification of chromosome inheritance modifiers in Drosophila melanogaster. Genetics 157:1623-37
Abstract	Faithful chromosome inheritance is a fundamental biological activity and errors contribute to birth defects and cancer progression. We have performed a P-element screen in Drosophila melanogaster with the aim of identifying novel candidate genes involved in inheritance. We used a "sensitized" minichromosome substrate (J21A) to screen approximately 3,000 new P-element lines for dominant effects on chromosome inheritance and recovered 78 Sensitized chromosome inheritance modifiers (Scim). Of these, 69 decreased minichromosome inheritance while 9 increased minichromosome inheritance. Fourteen mutations are lethal or semilethal when homozygous and all exhibit dramatic mitotic defects. Inverse PCR combined with genomic analyses identified P insertions within or close to genes with previously described inheritance functions, including wings apart-like (wapl), centrosomin (cnn), and pavarotti (pav). Further, lethal insertions in replication factor complex 4 (rfc4) and GTPase-activating protein 1 (Gap1) exhibit specific mitotic chromosome defects, discovering previously unknown roles for these proteins in chromosome inheritance. The majority of the lines represent mutations in previously uncharacterized loci, many of which have human homologs, and we anticipate that this collection will provide a rich source of mutations in new genes required for chromosome inheritance in metazoans.
Links	PubMed PMC1461595
Keywords	Animals; Cytoskeletal Proteins; DNA Transposable Elements; Drosophila Proteins; Drosophila melanogaster/genetics; Eukaryotic Initiation Factor-4E; Eye Proteins/genetics; Female; Genes, Insect; Glycoproteins/genetics; Histones/genetics; Homeodomain Proteins/genetics; Insect Proteins/genetics; Male; Membrane Glycoproteins/genetics; Microfilament Proteins; Microtubule-Associated Proteins/genetics; Peptide Initiation Factors/genetics; Protein Kinases/genetics; RNA-Binding Proteins; Trans-Activators/genetics; Transcription Factors; rab5 GTP-Binding Proteins/genetics; ras GTPase-Activating Proteins/genetics
edit table

Annotations

Gene product	Qualifier	GO ID	GO term name	Evidence Code	with/from	Aspect	Notes	Status
edit table

References

See Help:References for how to manage references in GONUTS.