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PMID:11226178

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Citation

Behrens, S, Maier, R, de Cock, H, Schmid, FX and Gross, CA (2001) The SurA periplasmic PPIase lacking its parvulin domains functions in vivo and has chaperone activity. EMBO J. 20:285-94

Abstract

The Escherichia coli periplasmic peptidyl-prolyl isomerase (PPIase) SurA is involved in the maturation of outer membrane porins. SurA consists of a substantial N-terminal region, two iterative parvulin-like domains and a C-terminal tail. Here we show that a variant of SurA lacking both parvulin-like domains exhibits a PPIase-independent chaperone-like activity in vitro and almost completely complements the in vivo function of intact SurA. SurA interacts preferentially (>50-fold) with in vitro synthesized porins over other similarly sized proteins, leading us to suggest that the chaperone-like function of SurA preferentially facilitates maturation of outer membrane proteins.

Links

PubMed PMC140197 Online version:10.1093/emboj/20.1.285

Keywords

Bacterial Proteins/metabolism; Carrier Proteins; Citrate (si)-Synthase/chemistry; Citrate (si)-Synthase/metabolism; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli Proteins; Genotype; Kinetics; Molecular Chaperones/metabolism; Peptidylprolyl Isomerase/chemistry; Peptidylprolyl Isomerase/genetics; Peptidylprolyl Isomerase/metabolism; Plasmids; Protein Folding; Ribonuclease T1/chemistry; Ribonuclease T1/metabolism; Sequence Deletion

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ECOLI:SURA

acts_upstream_of_or_within

GO:0061077: chaperone-mediated protein folding

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:SURA

involved_in

GO:0050821: protein stabilization

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:SURA

enables

GO:0051082: unfolded protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P02931
UniProtKB:P02932
UniProtKB:P02943

F

Seeded From UniProt

complete


See also

References

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