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PMID:11208127

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Citation

Durrbach, A, Raposo, G, Tenza, D, Louvard, D and Coudrier, E (2000) Truncated brush border myosin I affects membrane traffic in polarized epithelial cells. Traffic 1:411-24

Abstract

We investigate, in this study, the potential involvement of an acto-myosin-driven mechanism in endocytosis of polarized cells. We observed that depolymerization of actin filaments using latrunculin A decreases the rate of transferrin recycling to the basolateral plasma membrane of Caco-2 cells, and increases its delivery to the apical plasma membrane. To analyze whether a myosin was involved in endocytosis, we produced, in this polarized cell line, truncated, non-functional, brush border, myosin I proteins (BBMI) that we have previously demonstrated to have a dominant negative effect on endocytosis of unpolarized cells. These non-functional proteins affect the rate of transferrin recycling and the rate of transepithelial transport of dipeptidyl-peptidase IV from the basolateral plasma membrane to the apical plasma membrane. They modify the distribution of internalized endocytic tracers in apical multivesicular endosomes that are accessible to fluid phase tracers internalized from apical and basolateral plasma membrane domains. Altogether, these observations suggest that an acto-myosin-driven mechanism is involved in the trafficking of basolaterally internalized molecules to the apical plasma membrane.

Links

PubMed

Keywords

Actins/metabolism; Animals; Bicyclo Compounds, Heterocyclic/pharmacology; Biological Transport, Active; Caco-2 Cells; Cell Polarity; Chickens; Dipeptidyl Peptidase 4/metabolism; Endocytosis; Endosomes/metabolism; Epithelial Cells/metabolism; Humans; Microscopy, Immunoelectron; Microvilli/metabolism; Models, Biological; Myosins/chemistry; Myosins/genetics; Myosins/metabolism; Peptide Fragments/chemistry; Peptide Fragments/genetics; Peptide Fragments/metabolism; Recombinant Proteins/genetics; Recombinant Proteins/metabolism; Sequence Deletion; Thiazoles/pharmacology; Thiazolidines; Transferrin/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:TRFE

located_in

GO:0016324: apical plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:MYO1A

involved_in

GO:0016197: endosomal transport

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:MYO1A

involved_in

GO:0045056: transcytosis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:TRFE

part_of

GO:0016324: apical plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

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