PMID:11208127

Citation	Durrbach, A, Raposo, G, Tenza, D, Louvard, D and Coudrier, E (2000) Truncated brush border myosin I affects membrane traffic in polarized epithelial cells. Traffic 1:411-24
Abstract	We investigate, in this study, the potential involvement of an acto-myosin-driven mechanism in endocytosis of polarized cells. We observed that depolymerization of actin filaments using latrunculin A decreases the rate of transferrin recycling to the basolateral plasma membrane of Caco-2 cells, and increases its delivery to the apical plasma membrane. To analyze whether a myosin was involved in endocytosis, we produced, in this polarized cell line, truncated, non-functional, brush border, myosin I proteins (BBMI) that we have previously demonstrated to have a dominant negative effect on endocytosis of unpolarized cells. These non-functional proteins affect the rate of transferrin recycling and the rate of transepithelial transport of dipeptidyl-peptidase IV from the basolateral plasma membrane to the apical plasma membrane. They modify the distribution of internalized endocytic tracers in apical multivesicular endosomes that are accessible to fluid phase tracers internalized from apical and basolateral plasma membrane domains. Altogether, these observations suggest that an acto-myosin-driven mechanism is involved in the trafficking of basolaterally internalized molecules to the apical plasma membrane.
Links	PubMed
Keywords	Actins/metabolism; Animals; Bicyclo Compounds, Heterocyclic/pharmacology; Biological Transport, Active; Caco-2 Cells; Cell Polarity; Chickens; Dipeptidyl Peptidase 4/metabolism; Endocytosis; Endosomes/metabolism; Epithelial Cells/metabolism; Humans; Microscopy, Immunoelectron; Microvilli/metabolism; Models, Biological; Myosins/chemistry; Myosins/genetics; Myosins/metabolism; Peptide Fragments/chemistry; Peptide Fragments/genetics; Peptide Fragments/metabolism; Recombinant Proteins/genetics; Recombinant Proteins/metabolism; Sequence Deletion; Thiazoles/pharmacology; Thiazolidines; Transferrin/metabolism
edit table

Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
HUMAN:TRFE	located_in	GO:0016324: apical plasma membrane	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
CHICK:MYO1A	involved_in	GO:0016197: endosomal transport	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
CHICK:MYO1A	involved_in	GO:0045056: transcytosis	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
HUMAN:TRFE	part_of	GO:0016324: apical plasma membrane	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete

References

See Help:References for how to manage references in GONUTS.