GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:11201745
| Citation |
Michel, LS, Liberal, V, Chatterjee, A, Kirchwegger, R, Pasche, B, Gerald, W, Dobles, M, Sorger, PK, Murty, VV and Benezra, R (2001) MAD2 haplo-insufficiency causes premature anaphase and chromosome instability in mammalian cells. Nature 409:355-9 |
|---|---|
| Abstract |
The mitotic checkpoint protein hsMad2 is required to arrest cells in mitosis when chromosomes are unattached to the mitotic spindle. The presence of a single, lagging chromosome is sufficient to activate the checkpoint, producing a delay at the metaphase-anaphase transition until the last spindle attachment is made. Complete loss of the mitotic checkpoint results in embryonic lethality owing to chromosome mis-segregation in various organisms. Whether partial loss of checkpoint control leads to more subtle rates of chromosome instability compatible with cell viability remains unknown. Here we report that deletion of one MAD2 allele results in a defective mitotic checkpoint in both human cancer cells and murine primary embryonic fibroblasts. Checkpoint-defective cells show premature sister-chromatid separation in the presence of spindle inhibitors and an elevated rate of chromosome mis-segregation events in the absence of these agents. Furthermore, Mad2+/- mice develop lung tumours at high rates after long latencies, implicating defects in the mitotic checkpoint in tumorigenesis. |
| Links |
PubMed Online version:10.1038/35053094 |
| Keywords |
Anaphase; Animals; Antineoplastic Agents/pharmacology; Calcium-Binding Proteins/antagonists & inhibitors; Calcium-Binding Proteins/metabolism; Carrier Proteins; Cell Cycle Proteins; Cells, Cultured; Chromosome Aberrations; Chromosome Segregation; Fungal Proteins/antagonists & inhibitors; Fungal Proteins/metabolism; Gene Deletion; Genes, cdc; Humans; Karyotyping; Lung Neoplasms/genetics; Mice; Mitosis/genetics; Mitosis/physiology; Nocodazole/pharmacology; Nuclear Proteins; Tumor Cells, Cultured |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| edit table |
See also
References
See Help:References for how to manage references in GONUTS.
