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PMID:11090276

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Citation

Vincent, C, Duclos, B, Grangeasse, C, Vaganay, E, Riberty, M, Cozzone, AJ and Doublet, P (2000) Relationship between exopolysaccharide production and protein-tyrosine phosphorylation in gram-negative bacteria. J. Mol. Biol. 304:311-21

Abstract

The phosphorylation of proteins at tyrosine residues is known to play a key role in the control of numerous fundamental processes in animal systems. In contrast, the biological significance of protein-tyrosine phosphorylation in bacteria, which has only been recognised recently, is still unclear. Here, we have analysed the role in Escherichia coli cells of an autophosphorylating protein-tyrosine kinase, Wzc, and a phosphotyrosine-protein phosphatase, Wzb, by performing knock-out experiments on the corresponding genes, wzc and wzb, and looking at the metabolic consequences induced. The results demonstrate that the phosphorylation of Wzc, as regulated by Wzb, is directly connected with the production of a particular capsular polysaccharide, colanic acid. Thus, when Wzc is phosphorylated on tyrosine, no colanic acid is synthesised by bacteria, but when dephosphorylated by Wzb, colanic acid is produced. This process is rather specific to the pair of proteins Wzc/Wzb. Indeed, a much lesser effect, if any, on colanic acid synthesis is observed when knock-out experiments are performed on another pair of genes, etk and etp, which also encode respectively a protein-tyrosine kinase, Etk, and a phosphotyrosine-protein phosphatase, Etp, in E. coli. In addition, the analysis of the phosphorylation reaction at the molecular level reveals differences between Gram-negative and Gram-positive bacteria, namely in the number of protein components required for this reaction to occur.

Links

PubMed Online version:10.1006/jmbi.2000.4217

Keywords

Amino Acid Sequence; Bacterial Proteins; Escherichia coli Proteins; Gene Deletion; Gram-Negative Bacteria/enzymology; Gram-Negative Bacteria/genetics; Gram-Negative Bacteria/growth & development; Gram-Negative Bacteria/metabolism; Gram-Positive Bacteria/enzymology; Gram-Positive Bacteria/genetics; Gram-Positive Bacteria/metabolism; Membrane Proteins; Molecular Sequence Data; Phosphorylation; Phosphotyrosine/metabolism; Polysaccharides/biosynthesis; Protein Tyrosine Phosphatases/genetics; Protein Tyrosine Phosphatases/metabolism; Protein-Tyrosine Kinases/chemistry; Protein-Tyrosine Kinases/genetics; Protein-Tyrosine Kinases/metabolism; Sequence Alignment; Substrate Specificity

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ECOLI:WZB

acts_upstream_of_or_within

GO:0009242: colanic acid biosynthetic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:WZC

acts_upstream_of_or_within

GO:0009242: colanic acid biosynthetic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:WZB

involved_in

GO:0009242: colanic acid biosynthetic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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